KMD Bioscience Co., Ltd.
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2019-10-15 Hits(1216)
When the tumor cells are transferred from the original site to the other distal organs (cancer metastasis), most of the cancers are deadly, and whether the cancer cells will be transferred depends not only on the cells themselves but also with the microenvironment of the transfer habitat, Only a small portion of the cells that reach the new site can be successfully rooted and propagated, while the early process of promoting the growth of cancer cells at the second site is not clear at present, in part because of the lack of appropriate research tools. in a study published in Nature's Nature, an innovative technique that can be used to identify and isolate rare normal cells that can be in close contact with cancer cells that migrate to a new site, This approach helps to elucidate the early onset of interactions between the transfer cells and the nearby normal cells (contributing to the formation of cancer-transfer habitats).
The researchers engineered the mouse breast cancer cells to express a specific fluorescent protein, which contains an amino acid residue region that enables it to penetrate into the lipid layer, This characteristic allows the protein to be released from the cancer cells in a soluble form and absorbed by the adjacent cells, and the researchers have also studied a breast cancer metastatic model capable of expressing such a protein, At the same time, the researchers can use different fluorescent proteins to monitor the progression of cancer cells, and the researchers inject cancer cells into the mouse's stomach vein, and then the cancer cells will be colonized in the mouse lung tissue.After analyzing the lung tissue of mice, the researchers found that healthy cells located within the five layers of cancer cells could absorb protein, thus analyzing the specificity of healthy cells in close contact with tumor growth sites. The researchers found a direct correlation between the number of cancer cells in the lungs and the number of adjacent cells that ingest the protein, including immune cells, which can help breast cancer cells colonize in lung tissue. In previous studies, researchers have used other techniques to identify cells near malignant tumor tissues, such as those that specifically receive vesicles released by tumor cells, while the researchers have used new techniques to mark any type of cells that may exist near the site of metastasis.
The lipid osmotic fluorescent protein is stable in recipient cells for only 48 hours, so the researchers' method can evaluate the initial changes at the transfer site over time, but it is not suitable for long-term tracking. Cancer cells can promote tumor growth by changing the local environment, such as promoting vascular formation to increase nutritional supply or cause changes to protect the tumor from the immune attack of the host body. Rare cancer cells can reproduce successfully at the distal end and usually promote tumor progress by changing the microenvironment, such as making normal cells deficient in metabolites to increase nutritional availability, or preparing micro-environments to promote tumor growth. Researchers use new techniques to identify and isolate healthy cells used for molecular analysis, such as RNA sequencing, while also tracking changes that may promote the formation of transfer habitats.
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