KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
| Catalog Number | COV363 |
|---|---|
| Product Name | Recombinant COVID-19/SARS-CoV-2 Spike RBD protein, mFc Tag |
| Alias | SARS-CoV-2 Spike RBD, 2019-nCoV S1, COVID-19 antigen, SARS-CoV-2 Spike Protein Receptor Binding Domain |
| Size | 100μg,500μg |
| Species | COVID-19/2019-nCoV |
| Expression Host | Mammalian Cells |
| Applications | Immunogen, WB, ELISA, POCT |
| Purity | ≥95% as determined by SDS-PAGE |
| Endotoxin | < 0.1EU per μg protein as determined by the LAL method. |
| Biological Activity | Immobilized recombinant human anti-2019-nCoV Spike RBD protein monoclonal antibody (Cat.#: MAV101) at 2μg/ml (100 μl/well) can bind 2019-nCoV Nucleocapsid Protein (Cat.#: COV364). The ED50 of Recombinant 2019-nCoV Spike RBD is 2.8 ng/ml. |
| Buffer | 0.01M PBS, pH7.4 |
| Purification | Ni+ Affinity & SEC-HPLC |
| Source | A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) was fused a mFc tag at N terminus was expressed from Mammalian cells can bind with ACE2. |
| Background | A novel severe acute respiratory syndrome (SARS)-like coronavirus (SARS-CoV-2) recently emerged and is rapidly spreading in humans, causing COVID-191,2. A key to tackling this pandemic is to understand the receptor recognition mechanism of the virus, which regulates its infectivity, pathogenesis and host range. SARS-CoV-2 and SARS-CoV recognize the same receptor—angiotensin-converting enzyme 2 (ACE2)—in humans. Researchers determined the crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 (engineered to facilitate crystallization) in complex with ACE2. In comparison with the SARS-CoV RBD, an ACE2-binding ridge in SARS-CoV-2 RBD has a more compact conformation; moreover, several residue changes in the SARS-CoV-2 RBD stabilize two virus-binding hotspots at the RBD–ACE2 interface. These structural features of SARS-CoV-2 RBD increase its ACE2-binding affinity. Additionally, we show that RaTG13, a bat coronavirus that is closely related to SARS-CoV-2, also uses human ACE2 as its receptor. |
| Storage | Store the lyophilized powder at 4°C for one week and -20°C to -80°C for long time. Store the liquid form at -80°C. Avoid multiple freeze/thaw cycles. |
| Shipping Condition | The lyophilized powder will be shipped on ice packs. |
| Note | This product is used for research use only. Not for human or diagnostic use. |
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
