KMD Bioscience Co., Ltd.
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Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
| Catalog Number | COV366 |
|---|---|
| Product Name | Recombinant COVID-19/SARS-CoV-2 Spike Protein 1/S1-S2 protein |
| Alias | 2019-nCoV S1-S2 domain protein, COVID-19 antigen |
| Size | 100μg,500μg |
| Species | COVID-19/2019-nCoV |
| Expression Host | Mammalian Cells |
| Applications | Immunogen, WB, ELISA, POCT |
| Purity | ≥92% as determined by SDS-PAGE |
| Endotoxin | < 0.1EU per μg protein as determined by the LAL method. |
| Biological Activity | Immobilized recombinant human anti-2019-nCoV NTD monoclonal antibody (Cat.#: MAV108) at 2μg/ml (100 μl/well) can bind 2019-nCoV S1-S2 Protein (Cat.#: COV366). |
| Buffer | 0.01M PBS, pH7.4 |
| Purification | Ni+ Affinity & SEC-HPLC |
| Source | A DNA sequence encoding SARS-CoV-2 S1-S2 was expressed in mammalian system with 6*His tag at the N-terminus. |
| Background | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV. |
| Storage | Store the lyophilized powder at 4°C for one week and -20°C to -80°C for long time. Store the liquid form at -80°C. Avoid multiple freeze/thaw cycles. |
| Shipping Condition | The lyophilized powder will be shipped on ice packs. |
| Note | This product is used for research use only. Not for human or diagnostic use. |
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
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