KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
| Catalog Number | KMP4827 |
|---|---|
| Product Name | HCoV-HKU1 (isolate N5) Spike Protein(ECD), His Tag |
| Product Description | The HCoV-HKU1 (isolate N5) (HCoV-HKU1) Spike Protein(KMP4827) is produced in Insect Cells. A DNA sequence encoding the human coronavirus HKU1 (isolate N5) (HCoV-HKU1) spike protein (S1+S2 ECD) (Q0ZME7.1) (Met1-Pro1295) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. |
| Molecular Name | HCoV-HKU1 Spike |
| Molecular Weight | 144.4 kDa |
| Alias | HCoV-HKU1 Spike, S Protein, HCoV-HKU1 Spike Protein |
| Size | 50ug, 100ug, 200ug |
| Species | HCoV-HKU1 |
| Host | Insect Cells |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Buffer | 20mM Tris, 500mM NaCl, 10% Glycerol, pH7.5 |
| Purification | Affinity purification |
| Background | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Note | This product is for research use only. |
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
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