Antibody Pairing-Custom ELISA Development Service

KMD Bioscience is able to provide clients with project-specific antibody screening and matching services based on GMP guidelines.

 

Antibody Pairing Service - ELISA Enzyme Kit Development

ELISA (Enzyme Linked Immunosorbent Assay) is a medium- and high-throughput screening technology based on solid-phase carriers. It is widely used to qualitatively and quantitatively detect the content of peptides, proteins, antibodies and hormones in samples. In an ELISA assay, the antibody or antigen is pre-bound to the ELISA plate (96-well plate) and labeled with horseradish peroxidase (HRP), alkaline phospholipase (AP) or other chemiluminescent groups. The antibody or antigen is subjected to a color reaction. As shown in Figure 1, there are four main ELISA methods: Sandwich ELISA, Competitive ELISA (detection of antigen), Indirect ELISA (assay antibody) or Competitive ELISA (detection of small molecule compound), and the latter three are more commonly used.

Fig1. ELISA Detection Methods

The development of ELISA (enzyme-linked immunoassay) kit mainly involves the screening of antibody pairs. The performance of the antibody, the difference of recognition sites directly determines the quality of the ELISA kit. After antibody pairing, the work to be verified includes the detection sensitivity, linear range, repeatability, specificity and thermal stability.

 

Service Contents and Deliverables:

*Project evaluation and experimental design

*Evaluation of the antibody quality: antibody concentration (determined by BCA or Lowery method), antibody titer (requires antigen provided by the client)

*Antibody with matching array screening report (including N/P value)

*Detection sensitivity verification report

*Linear Range Verification Report

*Repetitive verification report

*Specificity report

*Thermal stability (37 °C or 25 °C thermal stability) report

*Molding kit and batch consistency data