KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
 |
KMD Bioscience has built up a mature antibody production platform with our well-established antibody engineering technology. With extensive experience in antibody production, we can offer comprehensive polyclonal antibody production services to the scientific community. Our capabilities enable us to provide complete support for alpaca, rabbit, goat, sheep and fully humanized antibody. Meanwhile, we can accept various antigens for polyclonal antibody production such as small molecules, peptides, modified peptides, proteins, fusion proteins, modified proteins, etc. |
KMD Bioscience has experienced immunology experts, standard operating procedures, and standard animal facilities, providing high-performance goat polyclonal antibody production services and related testing services. Moreover, we can ensure the ELISA titer is no less than 100,000 and all polyclonals we produced guarantee positive western blot result. At the same time, we also provide antigen customization services, including DNA synthesis, plasmid construction, protein expression, protein purification, protein modification, peptide synthesis, and small molecule structure analysis, etc.
Service Process:
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Service Content:
|
Procedure |
Description |
Timeline |
|
Immunization |
* Clients provide the antigens (small molecules, peptides, proteins); Purity>85%, concentration>0.5mg/mL, 3-5mg
* Immunize one goat, 4-5 boosts, ELISA titre determination
* Negative control: pre-immune serum collection, 1mL
* Serum harvest and ELISA titre determination
* Deliver ELISA report and 1 mL pre-immune serum |
12-14 weeks |
|
Consecutive bleed |
* Inject 500ug antigen mixed with 0.5mL Freund's incomplete adjuvant per goat
* 200-300mL blood is collected after every two weeks
* Ship the product & reports to investigator to test the serum and determine the quality of the antibodies. |
2-3 weeks/cycle |
|
Total bleed |
* Collect all the blood and the goat or sheep is humanely euthanized.
* Serum collection, provide the products according to investigator's requirements |
1-2 weeks |
|
Purification |
* Purify the goat/sheep antisera with Protein A (antigen affinity purification is also available)
* Deliver the purified polyclonal antibody to investigators |
1 week |
Besides, we can provide the following antibody services:
--Antibody Identification: ELISA, WB, IF, IP, IHC, SPR, etc.
--Antibody Modification: Enzyme modification (HRP, AP, biotinylation), fluorescent labeling (FITC, FAM, etc.), fusion label (GST, His), magnetic beads labeling, quantum dot labeling, etc.
Deliverable:
|
Materials |
Description |
|
Serum |
* 1mL pre-immune serum and 250-500mL antisera |
|
Antibody |
* DEAE purified antibody: 20-40mg
(10-20mg purified antibody will be delivered if investigator choose antigen affinity purification) |
|
Report |
* Immunogen sequence, SDS-PAGE result, expression & clone sequencing report, ELISA titre result
* According to the investigator's requirements, we can also provide the validation result of WB, IHC, IP, IF |
Service Highlights:
--Rich experience in polyclonal antibody development
--High affinity and specificity
--Large scale production capability
--Strict quality assurance: cGMP management rules
--Guaranteed positive western blot result and high ELISA titer
--A variety of antibody purification techniques are available
--One-stop services, including antigen production, antibody development, antibody purification, titer determination and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
