Antibody Platform

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Goat Polyclonal Antibody Production Service

KMD Bioscience has experienced immunology experts, standard operating procedures, and standard animal facility, which can provide first-class antibody products and technical services to scientists and researchers. In the meanwhile, our R&D staffs are good at antibody production of small molecule antigens, and with our unique technologies we are able to deal with the coupling and immunoassay of pesticides, small molecule compounds and heavy metal antigens.

Polyclonal Antibody Production Services- Goat Polyclonal Antibody Production Services

KMD Bioscience can guarantee the ELISA titer is no less than 100,000 for any goat polyclonal antibody we produced from any antigens or proteins (5-10 mg, purity>85%). We accept small molecules, peptides, modified peptides, proteins, fusion proteins, modified proteins, etc. used as antigens for goat polyclonal antibody production. At the same time, we also provide antigen customization services, including DNA synthesis, plasmid construction, protein expression, protein purification, protein modification, peptide synthesis, peptide modification, peptide conjugation, small molecule structure analysis and conjugation with other carrier proteins.

 

Custom Goat Polyclonal Antibody Service Protocol:

Procedure

Description

Timeline

Immunization

1. Clients provide the antigens (small molecules, peptides, proteins); Purity>85%,, concentration>0.5mg/mL, 3-5mg

2 Immunize one goat, 4-5 boosts, ELISA titre determination

3. Negative control: pre-immune serum collection, 1mL

4. Serum harvest and ELISA titre determination

5. Deliver ELISA report and 1 mL pre-immune serum

6-8 weeks

Consecutive bleed

1. Inject 500ug antigen per goat

2. 200-300mL blood is collected after every two weeks

3. Ship the product & reports to investigator to test the serum and determine the quality of the antibodies.

2-3 weeks/cycle

Total bleed

1. Collect all the blood and the goat or sheep is humanely euthanized.

2. Serum collection, provide the products according to investigator’s requirements

1-2 weeks

Purification

1. Purify the goat/sheep antisera with Protein A (antigen affinity purification is also available)

2. Deliver the purified polyclonal antibody to investigators

1 week

 

Besides, we can provide the following antibody services:

*Antibody Identification: ELISA, WB, IF, IP, IHC, SPR, etc.

*Antibody Modification: Enzyme modification (HRP, AP, biotinylation), fluorescent labeling (FITC, FAM, etc.), fusion label (GST, His), magnetic beads labeling, quantum dot labeling, etc.

 

Deliverable:  

Materials

Description

Serum

Pre-immune serum 1mL and antisera 250-500mL

Antibody

Protein G/A purified antibody: 20-40mg

(10-20mg purified antibody will be delivered if investigator choose antigen affinity purification)

Report

Immunogen sequence, SDS-PAGE result, expression & clone sequencing report, ELISA titre result

According to investigator’s requirements, we can also provide the validation result of WB, IHC, IP, IF