Human Monoclonal Antibody Production Services

There are two methods to produce human monoclonal antibody: Recombinant monoclonal antibody produced from phage display technology; Hybridoma cell line production by using cell fusion technology. We provide antigen design, synthesis and modification, in vitro sensitization, phage library construction and screening, antibody modification and application verification.

 

Monoclonal Antibody Production Services-Human Monoclonal Antibody Production Services 

After human body was infected by foreign substances such as viruses, bacteria and parasites, there are lymphocytes in the peripheral blood that are capable of secreting antibodies against these foreign substances. With our extensive antibody production experiences, we developed a unique platform to manufacture human monoclonal antibody. It is well known that EBV virus can infect and transform human cells, and the transformed human cells have the characteristics of proliferation of tumor cells in vitro. At the same time, using peripheral blood mononuclear cells to perform in vitro sensitization, our engineers used isolated and sensitized peripheral blood mononuclear cells infected by EBV virus, forming a unique sensitized and screened monoclonal cells strategy. This strategy can improve the success rate of antigen specificity and high-affinity human monoclonal antibody production.

 

Human monoclonal antibody production via our hybridoma-like technology: clients can provide small molecules, peptides, modified peptides, proteins, fusion proteins, modified proteins as antigens for monoclonal antibody production. Meanwhile, we provide antigen custom services as well, such as DNA synthesis, plasmid construction, protein expression, protein purification, protein modification, peptide synthesis, peptide modification, peptide and carrier protein cross-linking, small molecule structure analysis and carrier proteins conjugation.

 

Human Monoclonal Antibody Production Service Protocol:

Procedure

Description

Timeline

Peripheral blood mononuclear cell isolation and in vitro sensitization

1. Collecting peripheral blood from special populations and purifying monocytes

2. Culture monocytes in vitro and perform antigen sensitization

2-3 weeks

Hybridoma Production

1. EBV virus recovery and expansion

2. Monocyte immortalization: infection of cell cultures with EBV virus

3. Screening and cloning of hybridoma-like cells

4. After immunogen detection, select 1-10 positive clones, subcloning and expanded to 96-well plates

5. Screening positive subclones by ELISA and expansion of cultivation 

6. 1mL supernatant or positive clones delivered to investigator for testing

4-6 weeks

Functional Verification (Optional)

ELISA, WB, IF, FLAC, IHC, etc.

1-3 weeks

 

Service Highlights:

*High affinity and specificity—pM grade

*Antibodies produced from different sources: patients, rehabilitation and vaccine recipients

*Self-antibody and antigen development

*CAR-T