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Humanized Antibody Phage Library Services

KMD Bioscience provides quality custom phage display library construction and screening services for biological research and drug discovery related projects. We have extensive experience in phage display platform by employing various types of phage in various project objectives.

 

Human Antibody Phage Library

Phage display is a laboratory technique for the study of protein–protein, protein–peptide, and protein–DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. Natural human antibodies refer to collection of unimmunized human peripheral blood lymphocyte mononuclear cells, then after a series of gene cloning, the CDR regions of the antibody are spliced to the phage coat protein gene for display. Through repeated adsorption-elution-amplification processes, the phages containing high specificity and affinity antibody fragments are screened from the phage library. After enriching, amplifying and recombining to construct monoclonal antibodies, as shown in the following figure.

Types of Phage Display System

In general, M13 phage system is the most popular vehicle choice for phage display and extensively used in many types of research. We also provide phage display related services via either T4 or T7 phage systems, which can provide different promising alternatives by their special merits. In addition, clients can also provide us with antigens, then higher affinity and specificity of monoclonal antibodies can be obtained by sensitizing peripheral blood lymphocytes in vitro.

 

Service Highlights:

*Extensive experience: Extensive experience and strong track record in phage display platform.

*Large size of libraries: 107 - 1012 independent clones.

*High affinity: 10-7 - 10-13M.

*Various types of phage display systems: M13, T4, T7.

*Strict QC control.