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Humanized Antibody Phage Library Services

KMD Bioscience has been working on antibody research for many years. Antibody phage display library is a powerful tool for recombinant antibody preparation and antibody drugs development. KMD Bioscience has built up a mature antibody phage display platform, which can provide different levels and species of antibody phage display library for customers. Our researchers have extensive experience in phage display technology, and can construct phage display library with high capacity in a short period of time. We employ various types of phage in various project objectives. Meanwhile, we are devoted to providing comprehensive technical services including protein expression, antibody production, phage library construction, affinity analysis, and antibody affinity maturation.

 

Human Antibody Phage Library:

 

Phage display is a laboratory technique for the study of protein–protein, protein–peptide, and protein–DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. Natural human antibodies refer to collection of unimmunized human peripheral blood lymphocyte mononuclear cells, then after a series of gene cloning, the CDR regions of the antibody are spliced to the phage coat protein gene for display. Through repeated adsorption-elution-amplification processes, the phages containing high specificity and affinity antibody fragments are screened from the phage library. After enriching, amplifying and recombining to construct monoclonal antibodies, as shown in the following figure.

 

 

 

Types of Phage Display System:

 

In general, M13 phage system is the most popular vehicle choice for phage display and extensively used in many types of research. We also provide phage display related services via either T4 or T7 phage systems, which can provide different promising alternatives by their special merits. In addition, clients can also provide us with antigens, then higher affinity and specificity of monoclonal antibodies can be obtained by sensitizing peripheral blood lymphocytes in vitro.

 

Service Process:

 

 

Service Highlights:

 

--Extensive experience in antibody engineering development

--Guaranteed high affinity: 10-7 - 10-13M

--Multiple phage display systems: M13, T4, T7

--Large size of libraries: 107 - 1012 independent clones

--Strict quality control

--Various library searching strategies

--One-stop services, including antibody development, antibody sequencing, antibody labeling and related services