Pinpoint Targeted Integration Cell Line Services

Integrating exogenous plasmid DNA into the chromosome of host cells, and then express the target protein for a long time, which is called stable cell line. High-quality stable cell line plays an important role in biological research, including drug development, gene function research and recombinant antibody production. However, constructing an effective cell line is a complex and time-consuming task. KMD Bioscience has rich experience in stable cell line construction, our cell lines can be directly applied to downstream biological research. We have successfully constructed a large number of target cell lines for many clients so far. Based on advanced technology and dedicated research teams, KMD Bioscience can provide the following services:

 

CRISPR/Cas9 Knock-out Cell Line Services

IOS Overexpression Stable Cell Line Services

IOS RNAi Stable Cell Line Services

Pinpoint Targeted Integration Cell Line Services

 

Pinpoint Targeted Integration Cell Line Construction

When studying protein functional domains, it is necessary to mutate one or more bases accurately. Similarly, it is necessary to add a fluorescent protein or label to the N-terminus or C-terminus of the target protein when studying protein localization. KMD Bioscience can provides pinpoint targeted integration cell line services based on our advanced CRISPR/Cas9 system. The application of this platform is as follows:

 

* Gene marker: Adding a fluorescent protein or label to the N-terminus or C-terminus of the target protein.

* Pinpoint targeted mutation: Accurate mutation of one or more bases of the target gene.

* Pinpoint targeted integration: Any gene fragment can be integrated into any site of the genome.

 

Service Procedure:

 

Service Highlights:

*Customizable: We customize our offering to meet your specific project needs and provide experienced scientific support every step of the way.

*Extensive experience: Our scientists are experts at performing gene editing with CRISPR, from designing gRNA constructs for CRISPR to transfection and single clone generation of a wide range of cells.

*Pinpoint targeted integration rather than random integration.

*Stable inheritance, the gene will not be lost in the process of passage.

*Exogenous gene integration efficiency is not affected by fragment size.