KMD Bioscience Co., Ltd.

KMD Bioscience Co., Ltd. was established in Tianjin, China in 2014. The founder team has many years of experiences in human drug research and development. KMD Bioscience takes research & development as the foundation of its value and focuses on providing high-quality products and drug-antibody discovery services for global scientists, research institutes and pharmaceutical companies. As a high-tech enterprise in China, KMD Bioscience has  successfully obtained the ISO9001:2015 quality management system certification and national patent pilot laboratory  honor. At present, the company has more than 30 patents and 2 registered trademarks. KMD Bioscience provides more than 800+ technical service consulting, custom service design and project delivery for research institutes, hospitals, pharmaceutical companies and biological companies worldwide every year. At the beginning of its establishment, KMD Bioscience started from the phage display technology platform to provide high-quality antibody discovery services, and gradually established stable cell line construction and screening, natural protein extraction and fermentation, and recombinant protein custom expression services, a multi-species antibody discovery platform. Based on the above-mentioned major R&D platforms, KMD Bioscience has successfully developed many related reagents such as recombinant proteins, antibodies, antibody-drug similars, industrial enzymes, and diagnostic raw materials for scientific research and new drug discovery.

---Phage Antibody Display Technology Platform

KMD Bioscience utilizes its own phage display (M13, T7 phage display technology platform) to provide customers with cost-effective recombinant VHH antibody (Nanobody/Camel Nano Antibody) customization protocols, including animal immunization, library establishment, library screening, and in vitro expression of recombinant antibodies.

---Antibody Engineering Technology Platform

KMD Bioscience's antibody engineering platform includes monoclonal antibody production service platform, polyclonal antibody production service platform, and antibody sequencing and transformation platform.

Monoclonal Antibody Production Platform: Based on the hybridoma technology-based mouse monoclonal antibody technology platform, it can obtain high-affinity and high-specificity monoclonal antibodies in 2-3 months. At the same time, based on phage display technology, we can produce recombinant monoclonal antibodies from different species, including mouse, rabbit, camel, alpaca, and human monoclonal antibodies.

Polyclonal Antibody Production Service Platform: KMD Bioscience can provide rabbit polyclonal antibody, sheep polyclonal antibody, camel polyclonal antibody, bovine polyclonal antibody and other multi-species antibody preparation services. According to different antigen types, the senior antibody experts from KMD Bioscience will design the experiments to meet every customer's requirements.

Antibody Sequencing Platform: In order to meet the needs of a large number of clients for antibody gene sequence and amino acid sequence information, we provide full-length amino acid sequence analysis and N-terminal sequencing services of De Novo sequencing service.

Antibody Modification Technology Platform: KMD Bioscience's antibody engineering platform includes chimeric antibody preparation, antibody affinity maturation, and antibody humanization services. At present, we have successfully produced more than a dozen chimeric antibodies and humanized antibodies, which have been successfully delivered to the customers.

---Protein Expression and Purification Service Technology Platform

KMD Bioscience currently has four protein expression platforms that provide customers with comprehensive and superior protein expression technology services. 

Mammalian Expression Platform: KMD Bioscience currently has cell-free CHO, Expi CHO, CHO-DG44, HEK293F, Vero and other cell lines for protein expression production. We use Gibco cell culture medium and related transfection reagents to ensure the high quality and high expression level of each batch of mammalian cell protein expression products. At the same time, KMD Bioscience has an expression vector system that can provide customers with higher-purity proteins than the commonly used protein expression vectors on the market.

Insect Cell Expression Platform: At present, the insect-expressing cells commonly used by KMD Bioscience are SF9, and the medium is Gibco in order to provide customers with high-quality products. Using this protein expression platform, we have successfully prepared a variety of human transmembrane proteins, including a variety of GPCR family member proteins.

Prokaryotic Expression Platform: E. coli expression system has become very mature after years of development. However, an excellent expression strategy is essential if you want to obtain good soluble and high-yield protein expression in the E. coli expression system. KMD Bioscience's team of experts has massive experience and knowledge in protein expression and antibody production, and a very specific protocol will be provided to every customer.

Yeast Protein Expression Platform: Pichia pastoris, Saccharomyces cerevisiae and other protein expression systems, which are suitable for project requirements that require eukaryotic expression of protein, and it can ensure the yield and quality.

---Stable Cell Line Platform and Molecular Biology Platform

Stable Cell Line Construction Platform: KMD Bioscience can customize different types of stable cell line construction and screening services, ranging from multi-species, multi-cell line cell silencing, knockout, overexpression to other stable cells using Crisper/Cas9. At the same time, we could provide stable transfection cell line construction services based on electroporation or lipofection (Lipofectin2000/3000), such as CHO cell protein high expression cell lines.

Molecular Biology Platform of KMD Bioscience undertakes gene cloning, protein quantification, immunoblotting (SDS-PAGE, WB), host nucleic acid detection, antibody affinity purification, ELISA, endotoxin detection, HPLC analysis, Immunohistochemistry, IP, immune turbidity, flow cytometry and other related services.

KMD Bioscience has the experience and capabilities to partner with life science organizations to co-create antibodies and recombinant proteins as key reagents in drug discovery, in vitro diagnostics, and pharmaceutical companies. Moreover, we strive to continuously improve the customer experience through comprehensive technical support and program design and execution. Our mission and goal is very simple: make research easier and faster!