KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Antibody phage display library is a powerful tool for recombinant antibody preparation and antibody biosimilar development. KMD Bioscience knows that with quality in mind, you strive for efficiency. We have a mature antibody phage display platform, which can provide different levels and species of phage display antibody libraries for you. Our researchers have extensive experience in phage display technology and can construct phage display library with high capacity in a short period of time. KMD Bioscience uses various types of phage for multiple project objectives. Other services are provided to support your research, including protein expression, antibody production, phage display library construction, affinity analysis, phage display antibody screening and antibody affinity maturation.
KMD Bioscience phage display library construction service include: [1] Naive Human Antibody Libraries derived from healthy donors, available in scFv phage display, Fab phage display, VHH antibody library, and custom formats. [2] Non-Human Primate Immune Antibody Collections, accessible in scFv phage display, Fab phage display, VHH antibody library, and personalized formats for comparative research. [3] Comprehensive Synthetic Antibody Libraries, with options for scFv phage display, Fab phage display, VHH antibody library, and specific customizations to meet precise research needs.
Phage Display Antibody Library:
Phage display is a laboratory technique for the study of protein–protein, protein–peptide, and protein–DNA interactions that use bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. Natural human antibodies refer to a collection of unimmunized human peripheral blood lymphocyte mononuclear cells, then after a series of gene cloning, the CDR regions of the antibody are spliced to the phage coat protein gene for display. The phages containing high specificity and affinity antibody fragments are screened from the phage library through related adsorption-elution-amplification processes. After enriching, amplifying and recombining, monoclonal antibodies are constructed, as shown in the following figure.
Types of Phage Display System:
In general, the M13 phage display system is the most popular choice for phage display and is extensively used in many types of research. Phage display-related services via either T4 or T7 phage display systems are also provided to ensure you with various promising alternatives by their particular merits. You only need to provide the antigen, and higher affinity and specificity of monoclonal antibodies can be obtained by sensitizing peripheral blood lymphocytes in vitro under our platform.
Phage Display Library Construction Service Process:
[1] Phage display antibody Library source:
--Synthetic Libraries: Sequences are designed and synthesized in vitro to cover a broad range of sequence variability.
--Immune Libraries: Genetic material is extracted from the B cells of immunized animals or humans, capturing the diversity of the immune response.
--Naïve Libraries: Genetic material is obtained from unimmunized animals or humans, reflecting a broad, unselected repertoire of antibody sequences.
[2] Phage Display Library Construction Service Process:
--Preparation of the Antibody Fragment Gene
--Cloning into Phage Display Vector
--Transformation into Host Bacteria
--Packaging into Phage Particle
--Amplification and Harvesting
--Quality Control and Validation
--Phage display antibody screening or panning.
Phage Display Library Construction Service Highlights:
--Extensive experience in antibody engineering development
--Guaranteed high affinity: 10-7 - 10-13M
--Multiple phage display systems: M13, T4, T7
--Large size of libraries: 107 - 1012 independent clones
--Strict quality control
--Various library searching strategies
--One-stop services, including antibody development, antibody sequencing, antibody labeling and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
