KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
KMD Bioscience considers technology to be its heart and has refined the antibody production platform over the years, supported by advanced antibody engineering technology. Phosphorylation events are commonly probed by phosphorylation-specific antibodies. With many years of in-depth research in antibody phosphorylation, we can provide high-quality phosphorylated antibody production services, including synthesis of phosphorylated and non-phosphorylated peptides, conjugation to a carrier protein, immunization, serum production and affinity purification of the antibodies. Produce high-affinity antibodies that recognize this most widely studied post-translational modification, phosphorylation.
Quality service is not just a slogan at KMD Bioscience.
We have a well-established antibody platform, advanced antibody engineering technology and experienced scientists. Under these supports, KMD Bioscience can offer high-affinity antibodies in accordance with your requirements. Phosphorylation-specific polyclonal and monoclonal antibody production services based on traditional hybridoma and polyclonal antibody production technologies are also available.
Phosphorylated Antibody Production:
Protein phosphorylation is a significant post-translational modification (PTM) in cells. It mainly involves regulating the protein biological activity, including activation or inhibition of protein activity and ultimately regulating the cell signal transduction pathway. KMD Bioscience can provide phosphorylation-specific antibody production services based on the phage display platform.
Service Procedure:
|
Steps |
Specification |
Deliverables |
|
Antigen design and synthesis |
* Clients provide (phosphopeptide, non-phosphopeptide), purity >90% * Clients provide target antigen sequence (polypeptide or protein sequence and Phosphorylation site) * We design and synthesize phosphorylated or non-phosphorylated antigen peptides (Optional) |
(Antigen production by KMD Bioscience)
* MS and HPLC reports (for both phosphopeptide and non-phosphopeptide).
* 10mg phosphopeptide (>90% purity).
* 2-5mg non-phosphopeptide (>90% purity). |
|
Animal immunization |
* Protocol 1: Immunization of 5 Balb/c mouse (using a unique mouse immunoadjuvant to produce high titer antiserum rapidly in 20 days). Detection of serum titer by ELISA.
* Protocol 2: Immunization of 2-3 rabbits (using a unique rabbit immunoadjuvant to produce high titer antiserum rapidly in 28 days). Detection of serum titer by ELISA. (Other animals are available) |
* 1-2 ml of pre-immune serum.
* ELISA identification report. |
|
Production of antibody phage display libraries |
* Collecting B lymphocytes after immunization.
* mRNA extraction & cDNA synthesis & phage vector construction & transformation & amplification.
* Screening of antibody library with phosphorylated and non-phosphorylated antigens.
* Amplification of positive clones & Construction of shuttle plasmids.
* Small-scale and large-scale mammalian system expression (293, CHO). Antibody purification and identification. |
* Recombinant expression plasmid.
* 2-5 mg of purified recombinant antibody.
* ELISA results (both for phosphopeptide and nonphosphopeptide).
* Western Blot identification report (Optional). |
Service Instance:
Customer-tailored services are available. KMD Bioscience can provide custom phosphorylation-specific antibody production services in accordance with different requirements of our customers. Every product related to the process is under strict quality control to help your research.
Case 1:
Service Highlights:
--Extensive experience in antibody production and phage display platform
--Guaranteed high affinity and specificity
--Sequence diversity
--Multiple antibody expression validation options are available
--High ELISA titer: Guaranteed ELISA titer up to -104
--A variety of production modes: 5L, 15L, 80L fermenters for selection
--Strict quality assurance: GMP/GLP grade
--One-stop services, including antibody development, phage library construction, antibody modification and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
