KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
KMD Bioscience is experienced in antibody production research. By using phage antibody display technology, KMD Bioscience can provide comprehensive recombinant monoclonal antibody production services, including rabbit, mouse, camel, sheep monoclonal antibody generation. Our scientific team is highly qualified, full of enthusiasm and sincere, and we adopt standard operating procedures, which can construct high-quality immunized antibody libraries and screen out high affinity monoclonal antibodies. KMD Bioscience is right for you because we are not only competent but also considerate.
The rabbit antibody phage display library has many advantages, such as it can produce monoclonal antibodies with high specificity, high activity and high affinity in a short time. With extensive experience in phage display technology, KMD Bioscience provides high-quality rabbit monoclonal antibody production services to meet customers' research needs. Moreover, we provide a complete portfolio of services ranging from antigen design, synthesis and modification, animal immunization, library construction and screening to downstream services like antibody modification and application verification.
Recombinant Rabbit Monoclonal Antibody Generation:
The rabbit antibody phage display library is a good source of rabbit-derived polyclonal antibodies and monoclonal antibodies. Compared with hybridoma technology, hybridomas prepared by phage library technology can be stored on phagemid vectors in the form of antibody genes, combining the advantages of different protein expression systems, such as the use of cell-free expression systems, enabling animal-free production and obtaining high specificity and high affinity recombinant monoclonal antibody. The ability of rabbit monoclonal antibodies to recognize the common antigenic determinants of rodents and human antigens is much better than that of murine monoclonal antibodies, which is also due to the distant evolution of rat and mouse commonly used in rabbits and rodents. Rodent monoclonal antibodies do not affect the common antigenic determinants of rodents and human antigens, while rabbit antibodies often recognize the common antigenic determinants of rodents and human antigens, overcoming this obstacle in antibody production.
In the process of developing recombinant monoclonal antibodies by phage technology, KMD Bioscience utilizes rich experience and technology accumulation to construct chimeric antibodies by cloning rabbit antibody variable region (Vλ, Vk, and VH) into human antibody backbone monovalent protein to avoid the toxicity of rabbit Fab antibody fragments to E. coli. Subsequently, the highly specific and high affinity rabbit-derived antibody variable regions (Vλ, Vk, and VH) were cloned into a plasmid vector carrying a rabbit antibody scaffold protein. Combined with our recombinant protein expression platform, yields of up to 5g/L can be achieved with our transient expression.
Service Process:
Service Content:
|
Procedure |
Carrier Type |
Deliverable |
Lead Time |
|
Hapten Modification |
/ |
Modified small molecule derivatives
LC-MS result |
3-4 weeks |
|
Complete Antigen Production |
* Ovalbumin (OVA)
* limpet hemocyanin (KLH)
* Human Serum Albumin (HSA)
* Human gamma globulins |
Complete antigen with SDS-PAGE, UV or MALDI-TOF identification results |
1-2 weeks |
|
Phage Antibody Library Production |
* Antigen emulsification
* Immunization
* Collection of splenocytes (or peripheral blood lymphocytes), variable region gene isolation
* Phagemid construction, transfection expression, library construction
* Antibody screening, re-cloning, antibody expression |
PCR results, immunoassay results (including ELISA titer and specificity test) |
1-2 months |
|
Recombinant antibody production |
* Antibody screening, construction of expression plasmid, eukaryotic or prokaryotic expression of recombinant antibodies |
* Recombinant Antibody, expression plasmid, cloned strain, Experimental report |
1-2 months |
Service Highlights:
--Rich experience in recombinant monoclonal antibody development
--High affinity and specificity
--Sequence diversity
--Multiple antibody expression validation options are available
--Antibody sequence humanization service is available
--Mature antibody and recombinant protein platform
--Strict quality assurance: cGMP management rules
--Guaranteed positive western blot result
--Shorten the cycle of antibody development
--One-stop services, including antigen preparation, antibody development, phage library construction, antibody modification and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com