Recombinant Mouse Monoclonal Antibody Production Services

KMD Biosciences can provide two methods of production of mouse monoclonal antibodies: recombinant monoclonal antibody produced from phage display technology; hybridoma cell line production by using cell fusion technology. Our services range from antigen design, synthesis and modification, animal immunization, phage library construction and screening to antibody modification and application identification.

 

Recombinant Mouse Monoclonal Antibody Generation 

 

In the phage technique for the development of recombinant mAb, we cloned the variable regions of the murine antibody (Vλ, Vk, and VH) into the phagemid system with the M13 phage pill fusion protein, which was subsequently screened for high specificity and High-affinity rabbit-derived antibody variable regions (Vλ, Vk, and VH) were cloned into plasmid vectors carrying mouse antibody scaffold proteins and transiently expressed in our recombinant expression platform (up to ~5 g/L).

 

The production protocol is shown as follows:

  

Packages

Carrier Type

Deliverable

Lead time

Hapten Modification

/

Modified small molecule derivatives

LC-MS result

1-2 weeks

Complete Antigen Production

*Bovine serum albumin (BSA)

*Ovalbumin (OVA)

*Keyhole limpet hemocyanin (KLH)

*Human Serum Albumin (HSA)

*Human gamma globulins

Complete antigen with SDS-PAGE, UV or MALDI-TOF identification results

1-2 weeks

Phage Antibody Library Production

*Antigen emulsification

*Immunization

*Collection of splenocytes (or peripheral blood lymphocytes), variable region gene isolation

*Phagemid construction, transfection expression, library construction

*Antibody screening, re-cloning, antibody expression

PCR results, immunoassay results (including ELISA titer and specificity test)

1-2 months