C-Reactive Protein (CRP)

Serum C-reactive protein (CRP), heparin-binding protein (HBP), serum procalcitonin (PCT), interleukin 6 (IL-6), and serum amyloid A (SAA) are markers of inflammatory response. When acute infection and inflammation occur in the human body, the liver generates and simultaneously releases the acute phase reactant C-reaction protein (CRP). As one of the inflammation markers, CRP is an acute phase reactive protein with an isoelectric point of 5.5 and possesses excellent stability and good precision, which is usually used as one of the reference indicators for identifying bacterial or viral infections.

KMD Bioscience independently develops and produces C-reactive protein products, by immunizing mice with CRP antigen to obtain mouse anti-human CRP monoclonal antibody (detection) and (capture), with high specificity and small batch-to-batch variation, which supports researchers to carry out the activity research of human CRP protein targets and protein interaction research. And it can be applied to lateral flow immunochromatographic assay (LFIA), chemiluminescent immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA). KMD Bioscience offers a diverse range of protein products, including recombinant proteins, viral proteins and bacterial proteins, etc. All the protein products provided have undergone strict QC verification and are characterized by low endotoxin content and high purity. With an experienced R&D team and a mature quality management system, the platform can provide customized services according to customers' R&D needs, from gene sequence design, protein expression host selection, to monoclonal antibody product, to meet the needs of diagnostic raw material development, scientific research and other applications.

The inventory of reagents associated with serum C-Reactive Protein (CRP) that KMD Bioscience can offer:

Molecular Structure and Characteristics of CRP

C-Reactive Protein (CRP), an acute temporal protein, possesses an ortho-pentameric structure. As a member of the penetratin family, CRP has a highly conserved gene sequence and occupies chromosome 1, q23. It consists of identical non-glycosylated polypeptide subunits, five in number with two calcium binding sites and 206 amino acid residues each, and has a relative molecular mass of 115-140 kDa. The subunits are assembled non-covalently into a cyclic pentamer with a symmetric structure (very few proteins have a cyclic pentamer). symmetrical (very few proteins have such a configuration).

Figure 1 Schematic diagram of CRP molecular structure

CRP is present at very low levels in normal human serum, but when a bacterial infection occurs, the concentration of CRP increases dramatically, and its value exceeds the normal value by a factor of 1,000-2,000. At the same time, the more serious the degree of infection, the greater the increase in the concentration of CRP. The concentration drops rapidly after the inflammation is cured and returns to normal levels within a week. Surprisingly, when the body is infected by a virus, the concentration of CRP rises and falls insignificantly, and its changes are not affected by individual differences, the state of the body and therapeutic drugs.

Figure 2 Changes in CRP concentration in the body before and after infection

Biological function of CRP

C-reactive protein (CRP) is synthesized during inflammation, infection, and tissue injury through the stimulation of cytokines such as IL-6, IL-2, and TFN, and the liver is the main site of its synthesis.CRP exerts its effect during platelet-mediated cytovirus through two pathways: pathway 1: CRP is oxidized and activated by platelets at the same time; pathway 2: CRP reacts with platelet-activating factor (PAF). Reaction. Since PAF inhibits PAF-induced neutrophil binding by stimulating platelet aggregation and arachidonic acid release from platelet membranes, it mediates the important role of CRP in the inflammatory response.

As a supplier of raw material for in vitro diagnosis, KMD Bioscience is committed to the rapid development and large-scale production of proteins and antibodies for in vitro diagnostics, and establishes stable cell line construction and screening, natural protein extraction and fermentation, recombinant protein customized expression services, and multi-species antibody discovery platform. Based on the above platforms, KMD Bioscience successfully develops many recombinant proteins, antibodies, antibody drug target proteins, industrial enzymes, diagnostic raw materials and other related reagents for scientific research and new drug discovery. KMD Bioscience adheres to independent innovation and breakthroughs in key technologies, and has successively obtained the national patent pilot unit and laboratory ISO9001:2015 quality management system certification and adheres to the continuous optimization to effectively ensure the quality stability of the products in the production process and final delivery. All antibodies provided by KMD Bioscience are subject to stringent testing to ensure their purity and sensitivity for a variety of different diagnostic platforms, such as LFIA, ELISA, CLIA, POCT and so on.