Interleukin (IL-6)

Inflammatory markers include interleukin-6 (IL-6), serum C-reactive protein (CRP), serum calcitoninogen (PCT), heparin-binding protein (HBP), and serum amyloid A (SAA). Interleukin-6 (IL-6) belongs to the interleukin family and is a glycosylated protein. It is a polypeptide chain consisting of 184 amino acids with a molecular weight of 26 KD. IL-6 plays a central regulatory function in the process of infection and inflammation, and is one of the important inflammatory response factors. As a pleiotropic cytokine, IL-6 can be synthesized and secreted by various types of cells, including T cells, fibroblasts, monocytes, and endothelial cells involved in various cellular functions.

As a supplier of in vitro diagnostic raw materials, KMD Bioscience is committed to the rapid development and large-scale production of proteins and antibodies for in vitro diagnostics, and establishes stable cell line construction and screening, natural protein extraction and fermentation, recombinant protein customized expression services, and multi-species antibody discovery platform. Based on the above platforms, KMD Bioscience successfully develops many recombinant proteins, antibodies, antibody drug target proteins, industrial enzymes, diagnostic raw materials and other related reagents for scientific research and new drug discovery. KMD Bioscience adheres to independent innovation and breakthroughs in key technologies, and has successively obtained the national patent pilot unit and laboratory ISO9001:2015 quality management system certification and adheres to the continuous optimization to effectively ensure the quality stability of the products in the production process and final delivery. All antibodies supplied by KMD Bioscience are rigorously tested to ensure their purity and sensitivity for use in a variety of different diagnostic platforms, such as LFIA, ELISA, CLIA, POCT, and so on.

The inventory of reagents associated with Interleukin 6 (IL-6) that KMD Bioscience can offer:

Structure of IL-6

Interleukin-6 (IL-6) is a small molecule glycoprotein with a molecular weight of 19-28 kDa, consisting of 184 amino acids in four alpha helices, usually in monomeric form, and with an isoelectric point of 5.0.The gene encoding IL-6 is present on human chromosome 7p15-21, which contains an intron (4) and an exon (5).There are three receptor-binding sites, including one specific receptor, and one specific receptor for IL-6. IL-6 has three receptor binding sites, including one specific receptor IL-6R (IL-6 binding receptor protein, IL-6R) binding site, and two gp130. (signal-transducing protein) binding sites.

Figure 2 Schematic diagram of IL-6 protein structure

IL-6 signaling mode

Interleukin-6 (IL-6) exerts its effects through three different signaling modes: classical signaling, trans-signaling, and trans-presentation. When classical signaling occurs, IL-6 binds the complex of mIL-6R and gp130, which then activates the JAK-STAT3 pathway for signaling. When trans signaling occurs, IL-6 binds to sIL-6R in vivo. And in trans-presentation, mIL-6R on dendritic cells binds to IL-6 and is presented to T cells expressing gp130 on the surface.This alternation of IL-6 signaling is an important event in the initiation of helper T cell 17 (Th17).

Biological role of IL-6 in inflammation and immunity

The concentration of interleukin-6 (IL-6) in the circulation of a normal organism is extremely low, about 1-5 pg/ml. when an inflammatory response occurs, the concentration of IL-6 rises dramatically, and in the case of sepsis, it can reach the ug/ml concentration range. In the early stages of inflammation, after IL-6 is produced at the local lesion site, it first enters the liver through the blood circulation, followed by the rapid induction of the production of acute temporal proteins, such as CRP, FGG, SAA, and HP.On the other hand, IL-6 decreases the synthesis of fibronectin, albumin, and transferrin. When the body is exposed to high levels of SAA for a long period of time, the formation of amyloid A causes serious complications in chronic inflammatory diseases.

KMD Bioscience independently develops and produces interleukin 6 (IL-6) protein products, by immunizing mice with IL-6 antigen to obtain mouse anti-human IL-6 monoclonal antibody (detection) and (capture), which has a high specificity and a small batch-to-batch variation, and supports researchers to carry out the activity research of IL-6 protein targets and protein interaction research. And it can be applied to lateral flow immunochromatographic assay (LFIA), chemiluminescent immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA). KMD Bioscience offers a diverse range of protein products, including recombinant proteins, viral proteins and bacterial proteins, etc. All the protein products provided have undergone rigorous QC validation and are characterized by low endotoxin content and high purity. With an experienced R&D team and mature quality management system, the platform can provide customized services according to customers' R&D needs, from gene sequence design, protein expression host selection, to monoclonal antibody production in every R&D process, to meet the needs of diagnostic raw material development, scientific research and other different applications