KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Integrating exogenous plasmid DNA into the chromosome of host cells, and then expressing the target protein for a long time is called a stable cell line. A high-quality stable cell line plays an essential role in biological research, including biosimilar development, gene function research and recombinant antibody production. However, constructing an effective cell line is a complex and time-consuming task. KMD Bioscience is experienced in stable cell line construction. Combining advanced instrumentation and skilled scientists, KMD Bioscience can provide you with a high-quality cell line construction service. Cell lines constructed by KMD Bioscience can be directly applied to downstream biological research. We have successfully constructed a large number of target cell lines for many research clients. Based on advanced technology and dedicated research teams, KMD Bioscience can provide the following services:
CRISPR/Cas9 Knock-out Cell Line Services
IOS Overexpression Stable Cell Line Services
IOS RNAi Stable
Pinpoint Targeted Integration
CRISPR/Cas9 Knock-out Cell Line Construction:
CRISPR/Cas9 is a powerful technique for genome manipulation and causing a buzz in the science world. It has the potential to alter the course of research and drug discovery by providing scientists with a powerful tool to change any gene in any cell in a highly targeted manner and without introducing foreign DNA. In the Type II CRISPR System (Fig 1), CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA) form a complex through base pairing that can specifically recognize genomic sequences. Then the PAM (5'-NGG-3') sequence binds and invades DNA, directing the Cas9 endonuclease to generate double-strand breaks (DSBs) in the target fragment. This complex can be simplified by fusing crRNA and tracrRNA sequences to form single-guided RNA (sgRNA). The fused RNA has similar activity to wild-type and is more convenient for researchers because of its simplified structure. By connecting the elements of sgRNA and Cas9, it is possible to obtain a plasmid that can express both of them simultaneously. It can then be transfected into cells to manipulate the target gene.
Fig 1. Schematic representation of CRISPR/Cas9-mediated genome editing
Service Procedure:
Service Highlights:
--Customizable: We customize our offering to meet your specific project needs and provide professional scientific support every step of the way.
--Extensive experience: Our experts are good at performing gene editing with CRISPR, from designing gRNA constructs for CRISPR to transfection and single clone generation of a wide range of cells.
--Higher knock-out efficiency.
--We can knock out multiple genes on the same cell.
--We can provide a variety of expression vectors with different fluorescent and resistance markers, making it easier to screen for successful knock-out cells.
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806.
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com