KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
| Catalog Number | COV364 |
|---|---|
| Product Name | Recombinant COVID-19/SARS-CoV-2 Nucleocapsid Protein |
| Alias | 2019-nCoV NP domain protein, COVID-19 antigen |
| Size | 100μg,500μg |
| Species | COVID-19/2019-nCoV |
| Expression Host | E.coli |
| Applications | Immunogen, WB, ELISA, POCT |
| Purity | ≥95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Biological Activity | Immobilized recombinant human anti-2019-nCoV NP monoclonal antibody (Cat.#: MAV106) at 2μg/ml (100 μl/well) can bind 2019-nCoV Nucleocapsid Protein (Cat.#: COV364). The ED50 of Recombinant 2019-nCoV Nucleocapsid Protein-His(Cat.#: COV364) is 32 ng/ml. |
| Buffer | 0.01M PBS, pH7.4 |
| Purification | Ni+ Affinity & SEC-HPLC |
| Source | A DNA sequence encoding Met1-Ala419 of SARS-CoV-2 Nucleocapsid was expressed in E. coli system with 6*His tag at the N-terminus. The DNA residue <100pg/5ug. |
| Background | Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Storage | Store the lyophilized powder at 4°C for one week and -20°C to -80°C for long time. Store the liquid form at -80°C. Avoid multiple freeze/thaw cycles. |
| Shipping Condition | The lyophilized powder will be shipped on ice packs. |
| Note | This product is used for research use only. Not for human or diagnostic use. |
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
