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Tag antibodies are highly specific monoclonal and polyclonal antibodies for the detection and purification of the tagged proteins. Epitope tags are small peptides that are fused to the protein of interest using recombinant DNA techniques. They make it possible to study recombinant proteins when protein-specific antibodies are not available or not suitable for your experimental design. |
A tag antibody can be used for the detection and purification of multiple proteins with the same tag. Some tags can be removed from a tagged protein after purification by a specific protease. Some purposes of these tags are as follows.
Affinity tags can help particular proteins be purified from their crude biological source. Because they can be attached to these proteins, so it is easy to purify proteins by affinity techniques.
Solubilization tags can be used for recombinant proteins expression, particularly in special species like E. coli. Furthermore, aiding within the correct folding in proteins and preventing them from precipitating. Additionally, lots of affinity tags carry a dual role which can be used as a solubilization agent.
Fluorescence tags can be applied to offer visual readout on a protein.
Chromatography tags are applied to transform chromatographic properties of the protein to offer diverse resolutions across a selected separation method. Generally, these are composed of polyanionic amino acids and FLAG-tag is a very important tag of them.
KMD Bioscience offers a full range of epitope tag antibodies with high-quality to satisfy researchers' needs, including HA Tag antibodies, Myc Tag antibodies, DYKDDDDK Tag antibodies, GFP Tag antibodies and V5 Tag antibodies, etc. All of these tag antibodies are fully validated in various applications, such as ELISA, Western Blotting (WB), Flow Cytometry (FCM), Immunoprecipitation (IP) and Immunofluorescence (IF).
All the antibody products we provide have passed strict QC verification to ensure high purity and quality. Please feel free to call our sales representatives to get the technical information and quotation!