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Recombinant Human FGF Basic/FGF2 Protein, No Tag

Product Information
Catalog Number KMH1085
Product Name Recombinant Human FGF Basic/FGF2 Protein, No Tag
Product Description Recombinant Human FGF Basic/FGF2 Protein, No Tag (KMH1085) contains the human FGF2(Gly132-Ser288) was fused without Tag and expressed in E. coli.
Synonym basic Fibroblast Growth Factor, BFGF, FGFB, FGF 2, HBGF 2, fibroblast growth factor 2
Size 50ug, 500ug, 1mg
Species Human
Host E.coli
Uniprot ID P09038-4
Sequence GTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPD GRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLAS KCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPG QKAILFLPMSAKS
Tag No Tag
Predicted N-terminus Met
Purity > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Endotoxin Less than 0.001 ng/ug (0.01 EU/ug) as determined by LAL test.
Buffer Lyophilized from a 0.2 um filtered solution of 20mM Tris, 150mM NaCl, 3% Trehalose, 4% Mannitol, pH 7.5
Biological Activity Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 1.11 ng/ml. (Regularly tested)
Background FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.The family constitutes a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Storage Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Note This product is for research use only.