KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
| Catalog Number | KMH1174 |
|---|---|
| Product Name | Recombinant Human GM-CSF/CSF2 Protein, No Tag |
| Product Description | Recombinant Human GM-CSF/CSF2 Protein, No Tag (KMH1174) contains the human CSF2(Ala18-Glu144) was fused without Tag and expressed in E. coli. |
| Synonym | Granulocyte-Macrophage Colony Stimulating Factor, CSF, GMCSF, colony stimulating factor 2 |
| Size | 100ug, 500ug, 1mg |
| Species | Human |
| Host | E.coli |
| Uniprot ID | P04141 |
| Sequence | APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDL QEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCAT QIITFESFKENLKDFLLVIPFDCWEPVQE |
| Molecular Weight | The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result. |
| Tag | No Tag |
| Predicted N-terminus | Met |
| Purity | > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Buffer | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Biological Activity | Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). |
| Background | Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. |
| Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Note | This product is for research use only. |
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Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
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