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Catalog Number | KMH1256 |
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Product Name | Recombinant Human IFN-α2b/IFNA2 Protein, No Tag |
Product Description | Recombinant Human Interferon Alpha-2b is produced by our E.coli expression system and the target gene encoding Cys24-Glu188 is expressed. |
Synonym | INFα2a, Interferon alpha 2a, IFNA2, IFNA2a, human IFNA2a protein, Interferon alpha 2 protein, IFNA2 |
Size | 50ug, 500ug, 1mg |
Species | Human |
Host | E.coli |
Uniprot ID | P01563 |
Sequence | CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQF QKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLND LEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWE VVRAEIMRSFSLSTNLQESLRSKE |
Tag | No Tag |
Predicted N-terminus | Cys 24 |
Purity | > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Endotoxin | Less than 1EU per ug by the LAL method. |
Buffer | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Biological Activity | Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED50 for this effect is 5 ng/mL. |
Background | At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end. |
Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Note | This product is for research use only. |