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Catalog Number | KMP2349 |
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Product Name | Human CD279 Protein, His Tag |
Product Description | The Human CD279 Protein(KMP2349) is produced in HEK293 Cells and the target gene encoding Leu25-Gln167 is expressed with a 6His tag at the C-terminus. |
Molecular Weight | 16.78 kDa |
Alias | Programmed cell death protein 1 |
Species | Human |
Host | HEK293 Cells |
Size | 50ug, 100ug, 200ug |
Purification | Affinity purification |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | <1.0 EU/ug determined by the LAL method |
Buffer | PBS, pH7.4 |
Uniprot | Q9UM82 |
Function | Bridging factor that mediates the recruitment of CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis (PubMed:27307491, PubMed:27458237, PubMed:27545878, PubMed:27591049). Acts as a direct binding intermediate that bridges RNF31/HOIP, the catalytic subunit of the LUBAC complex, and the deubiquitinase (CYLD), thereby recruiting CYLD to the TNF-R1 signaling complex (TNF-RSC) (PubMed:27458237, PubMed:27545878, PubMed:27591049). Required to activate the 'Met-1'- (linear) and 'Lys-63'-linked deubiquitinase activities of CYLD (PubMed:27458237, PubMed:27591049). Controls the kinase activity of RIPK1 and TNF-alpha-induced necroptosis by promoting 'Met-1'-linked deubiquitination of RIPK1 by CYLD (By similarity). |
Background | Programmed cell death protein 1(PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. |
Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Note | This product is for research use only. |
References | 1.EMBO J. 35:1868-1884 (2016) 2.EMBO Rep. 17:1485-1497 (2016) 3.Cell Rep. 16:2271-2280 (2016) 4.Mol. Cell 63:990-1005 (2016) |