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Catalog Number | KMP4318 |
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Product Name | Rhesus c-Kit Protein, His Tag |
Product Description | The Rhesus c-Kit Protein(KMP4318) is produced in HEK293 Cells. A DNA sequence encoding the Rhesus KIT (NP_001253024.1) (Met1-Thr520) is expressed with a polyhistidine tag at the C-terminus. |
Molecular Name | c-Kit |
Alias | c-Kit Protein, Rhesus c-Kit Protein, His Tag |
Size | 50ug, 100ug, 200ug |
Species | Rhesus |
Host | HEK293 Cells |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | <1.0 EU/ug determined by the LAL method |
Buffer | PBS, pH7.4 |
Purification | Affinity purification |
Molecular Weight | 57 kDa |
Background | C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients. |
Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Note | This product is for research use only. |