KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
| Catalog Number | KMP4424 |
|---|---|
| Product Name | DENV (type 2, strain New Guinea C/PUO-218 hybrid) Envelope Protein, His Tag |
| Product Description | The DENV (type 2, strain New Guinea C/PUO-218 hybrid) Envelope Protein(KMP4424) is produced in Insect Cells. A DNA sequence encoding the Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E (AAC59274.1, with mutation Thr 76 Arg, Gln 77 Glu, Trp 101 Arg, Leu 107 Arg) (Met281-Gly679) was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | DENV Envelope |
| Molecular Weight | 45.7 kDa |
| Alias | DENV Envelope, E Protein, DENV Envelope Protein |
| Size | 50ug, 100ug, 200ug |
| Species | DENV |
| Host | Insect Cells |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Buffer | 20mM Tris, 500mM NaCl, pH8.0 |
| Purification | Affinity purification |
| Background | Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus. |
| Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Note | This product is for research use only. |
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
