KMD Bioscience Co., Ltd.
400-621-6806
2020-12-03 Hits(1071)
Monoclonal antibodies are identical immunoglobulins that are generated from identical immune cells, which have the advantages in specificity and low immunogenicity compared to traditional antibodies. They have been widely used in the research and application in the field of diagnosis and treatment therapies for various diseases. There are two most important antibody production technologies: hybridoma technology and phage display antibody library technology, and the two technologies have their own advantages.
Hybridoma Technology:
Hybridoma technology is a common method for the long-term production of large-scale monoclonal antibodies. Derived from the fusion of specific antibody-producing B lymphoblast with myeloma, the forming of the hybrid cell line is the key part of hybridoma technology. The general development of hybridoma cells starts with animal immunization and then the splenocytes would be harvested. The B cells that produce specific antibodies are isolated and in turn fused with myeloma. The obtained stable fusion cells are known as hybridoma cells, which can express antigen-specific monoclonal antibodies continuously. Monoclonal antibodies are of great value in basic research of biology and immunology. In addition, equipped with other advanced techniques, the extended application of monoclonal antibodies can further assist the development of either therapeutic or diagnostic agents.
Antibody Phage Display Library Technology:
Phage display is one of the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. This technology is mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages. Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.
Features:
In the field of antibody engineering, hybridoma technology and phage display antibody library technology are the two main antibody production technologies, which have their own advantages and disadvantages.
Features of Hybridoma Technology:
*High specificity, high affinity, and high accuracy
*Diverse species selection
*Cell fusion technology is simple and stable
Compared with the traditional hybridoma method, antibody phage display library has distinct advantages on discovering novel monoclonal antibodies and even the fully human antibody.
Features of Phage Display Technology:
*Large library capacity from 107 to over 1012
*High affinity from 10-7 to 10-9
*Various phage display systems (M13, T4, T7)
*Animal-free: The process does not require use of animals, in vitro production can be implemented by using the identical DNA and protein sequence.
*Decreased production time: Using protein expression system, the production time of antibody production can be weeks compared to months for hybridoma technology.
KMD Bioscience has been working on antibody research for many years. We have mature hybridoma technology, with our complete antibody platform, we can offer multi-species antibody production services by using antibody phage display library technology. Meanwhile, we are devoted to providing one-stop technical services including antibody purification, antibody labeling, affinity analysis, antibody humanization, antibody affinity maturation and related services.
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