KMD Bioscience Co., Ltd.
400-621-6806
2023-03-23 Hits(279)
Antibody phage display is a technique used to generate large libraries of antibodies that can bind to specific target molecules, such as proteins or small molecules. The technique involves genetically engineering bacteriophages, which are viruses that infect bacteria, to display antibodies on their surfaces.
To construct an antibody phage display library, the first step is to obtain genetic material that encodes the variable regions of antibodies. This can be done by isolating B cells from the immune system of an animal that has been immunized with the target molecule, or by using synthetic DNA sequences that encode random antibody variable regions.
The antibody variable regions are then cloned into a vector that contains the genetic sequence for a bacteriophage coat protein. This results in a library of bacteriophages, each of which displays a different antibody on its surface.
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The library is then subjected to a process called panning, in which the bacteriophages are exposed to the target molecule. The bacteriophages that bind to the target molecule are retained, while the non-binding bacteriophages are washed away. The retained bacteriophages can then be amplified and subjected to additional rounds of panning to increase the specificity and affinity of the antibodies.
Once the desired antibodies have been identified, the genetic material encoding the variable regions can be isolated and cloned into a vector that can be used for large-scale production of the antibody. The resulting antibody can then be used for a variety of applications, such as diagnostic assays, therapeutic treatments, or research tools.
Overall, antibody phage display library construction is a powerful technique for generating large and diverse libraries of antibodies that can be used for a wide range of applications.
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