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2024-06-19 Hits(333)
CRISPR/Cas9 Technology
On June 7, 2024, researchers from the Institute of Genomic Biology at the University of California, Berkeley successfully increased gene expression in rice using CRISPR-Cas9 technology. This result, published in the journal Scientific Progress, has attracted a lot of attention and may provide a reference for future strategies for editing super allelic genes.
During the research process, the author induced multiple CRISPR-Cas9 mutations using noncoding sequences upstream of rice genes. Through high-throughput screening, the author isolated 120 gene-edited alleles (from knockout to overexpression). The results showed that the protein expression abundance increased by two to three times, and the increase in protein abundance would improve water use efficiency. The authors also identified the role of 5 'UTR insertion/deletion and inversion in driving knockout/knockdown and overexpression phenotypes. The complex structural variation demonstrated the potential of CRISPR-Cas9 to promote genomic change and drive the pursuit of gene-edited non-transgenic rice plants, which accelerated the relaxation of their photoprotective effects.
The main author of the study, Dhruv Patel Tupper, said, "As our understanding of basic biology deepens, the potential of gene editing solutions in addressing agricultural and pharmaceutical challenges is also increasing. CRISPR-Cas9 expands its ability to target functional mutations, not only knocking out or weakening gene expression but also fine-tuning crop gene expression."
Dhruv Patel Tupper added that his method does not damage other basic processes of rice growth after maximizing DNA reversal. Although the team has demonstrated the feasibility of their research method, it is still relatively rare. Approximately 1% of the plants they cultivate have the desired phenotype, which is more difficult than using transgenic plant methods, and whether it can be further utilized in other plants requires some time and method optimization.
Reference
Dhruv Patel-Tupper, Armen Kelikian, Anna Leipertz, Nina Maryn, Michelle Tjahjadi, Nicholas G. Karavolias, Myeong-Je Cho, Krishna K. Niyogi. Multiplexed CRISPR-Cas9 mutagenesis of rice PSBS1 noncoding sequences for transgene-free overexpression. Science Advances, 2024; 10 (23) DOI: 10.1126/sciadv.adm7452
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