Affinity Measurement Service

 

Affinity is a characteristic parameter to evaluate the strength of the interaction between two molecules in the process of reversible reaction. It is an important indicator to understand molecules, identify biological processes, and discover and screen drugs. It is of great significance in early drug research and development, screening and identification, as well as quality control in downstream production, and is also widely used in basic research of life science, biopharmaceutical development and other fields.

KMD Bioscience has been committed to the study of interaction between biomolecules for many years, and our scientific experts have accumulated many years of experience in interaction research and detection. With rich technical experience, we can provide customers with high-quality affinity measurement service. We insist on fine control of each step of experiments to ensure the smooth progress of customers' experiments. At the same time, the company has a rich product pipeline, which can realize the qualitative and quantitative analysis of protein, antibody and Fab fragment interactions.

 

 

Affinity Measurement Service

 

KMD Bioscience provides accurate and rapid affinity testing based on Octet and Biacore platforms, and provides unique molecular interaction analysis services based on customer requirements, including the commonly used detection techniques in the industry: Surface plasmon resonance (SPR binding assay) and Bio-Layer Interferometry (BLI assay) guarantee accurate, objective and reliable detection results. We focus on providing customers with high quality one-stop technical services to meet the experimental needs of different customers.

 

Affinity Measurement Types

Protocol

Bio-Layer Interferometry (BLI) Assay

* BLI assay can detect proteins, nucleic acids, small molecule drugs, antibodies, viruses, cell lines, and other samples. It has been widely used in protein structural target analysis, drug development and screening, and other life science research fields.

Surface Plasmon Resonance (SPR) Assay * KMD Bioscience can provide SPR assay for many types of samples, including but not limited to small molecules, proteins, nucleic acids, lipids, bacteria, viruses and whole cells. We can provide you with a variety of biomolecular information: binding specificity, concentration analysis, kinetics and affinity analysis, and thermodynamics
Competitive ELISA Assay * Competitive ELISA Assay can be used for protein, nucleic acid, peptides, nanomaterials and other molecular interaction analysis.

 

Sample Requirement of Affinity Measurement Service

 

Sample Types

Inevitable Requirement

Antibodies, proteins, peptides, small molecules of compounds, etc

* Buffer solution: PBS, HEPPS and other organic reagents, no Tris, EDTA or DDT;

* Protein sample (including antibody) : 200ug, concentration >0.5mg/ml;

* Polypeptide sample: 200ug, concentration >1mg/ml;

* Small molecule compound: 1mg, concentration >1mg/ml, purity >90%, soluble in 100%DMSO or water.

 

Affinity Measurement Service Highlights

 

--Rich experience in affinity and kinetics measurement

--Different types of binding affinity assay technologies,including: BLI assay, CHIP assay, CO-IP(co immunoprecipitation) assay, spr binding assay, competitive ELISA assay, Pull down assay, yeast two hybrid assay.

--Extremely high accuracy by professional operation and strict quality control

--High-throughput antibody affinity characterizations by using upgraded Octet systems

--Label-free measurement, no additional lead time

--One-stop downstream services, including gene synthesis, custom cloning, or mutagenesis

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-400-621-6806