Yeast Display Platform

KMD Bioscience is committed to providing comprehensive yeast surface display technology services. We utilize professional biotechnology platforms to provide strong technical support for the early discovery and development of antibody drugs. Our services cover a full range of solutions from antibody yeast display systems, yeast library construction to yeast antibody library screening. We specialize in providing high-affinity and high-specificity antibodies to our customers.

Yeast Antibody Display Technology (Yeast Display Technology) is realized by fusing the variable region of the antibody to the Aga2p protein on the surface of the yeast cell, and using Aga2p to bind stably to the Aga1p protein on the yeast cell wall. This technique, combined with flow cytometry and high-throughput sequencing, enables rapid screening of antibodies with high affinity and specificity from large-scale antibody libraries. It is widely used in the fields of early discovery of antibody drugs, drug target antibody development, and CAR-T/CAR-NK cell therapy, providing an efficient and precise screening platform for biopharmaceutical R&D.

KMD Bioscience is committed to building an efficient, accurate and customizable yeast display antibody system, as well as comprehensive and optimized yeast library construction and yeast antibody library screening services, to provide strong technical support and solutions for researchers and biopharmaceutical companies around the world.

Antibody Yeast Display System

In yeast surface display technology, target proteins or peptides are attached to the surface of yeast cells. Combined with flow cytometry and NGS sequencing technology, millions of antibody clones can be sequenced in a single experiment, and antibodies with high affinity for specific antigens can be rapidly screened. Antibody, as a key tool in life science research and drug development, its performance directly determines the success of the experiment and the efficacy of the drug. The antibody yeast display system independently developed by KMD Bioscience cleverly integrates antibody gene fragments onto the surface of yeast cells, and utilizes the strong growth and replication ability and genetic stability of yeast to realize the efficient expression and screening of antibodies. This innovative platform not only greatly shortens the cycle of antibody discovery, but also significantly improves the affinity, specificity and stability of antibodies, injecting new vitality into the development of antibody drugs.

• Efficient Expression: By optimizing the yeast expression system, we are able to achieve a high-density and homogeneous display of antibody fragments on the surface of yeast cells, ensuring that every yeast strain can be a potential antibody producer.

• Precise Screening: Combined with flow cytometry, high-throughput sequencing and other advanced technologies, we are able to rapidly screen antibody candidates with specific binding activity and stability to meet diverse needs from basic research to clinical applications.

• Flexible customization: According to customer needs, we provide one-stop service from gene synthesis, vector construction to yeast transformation and screening validation to ensure that each project can get a tailor-made solution.

Yeast Display Library Construction Service

Yeast library construction is an important foundation for yeast surface display technology and an indispensable part of antibody discovery and drug discovery. KMD Bioscience provides a full range of yeast library construction services based on our deep accumulation in the field of yeast biology.

• Diversified libraries: We can construct a variety of yeast antibody libraries including gene libraries, expression libraries, mutation libraries, as well as VHH antibody libraries, scFv antibody libraries, etc., to meet the needs of different research fields.

• High-efficiency transformation: Advanced methods of electro- and chemical transformation are used to ensure efficient and stable introduction of exogenous genes into yeast cells and construction of high-quality libraries.

• Customized services: According to the specific needs of customers, we provide full customized services from library design, gene synthesis, vector construction to library validation to ensure that each library can accurately meet the research objectives.

Yeast Display Library Screening Service: Accurate Screening for Accelerated Discovery

In the process of antibody drug discovery and development, constructing high-quality antibody libraries and screening them efficiently are key steps. KMD Bioscience yeast antibody library screening service, relying on advanced yeast surface display technology and abundant antibody library resources, provides customers with antibody library construction and screening capabilities mainly through yeast two-hybrid service.

• Wide coverage: Our antibody library covers a wide range of antibody sequences from human, mouse, camel, rabbit and sheep sources, covering a wide range of antigenic targets, ensuring that highly effective antibodies against specific antigens can be discovered.

• Efficient Screening: Using an automated screening system combined with advanced bioinformatics analysis, we are able to screen high-affinity and high-specificity antibody candidates from a massive antibody library in a short period of time, accelerating the drug development process.

• Quality control: A strict quality control system is implemented throughout the entire process of antibody library construction and screening to ensure that every step of the operation complies with international standards, providing customers with high-quality antibody candidates.

Yeast Display Library Service Process

Fig.1 Yeast Antibody Library Construction Screening Flowchart

Yeast Display Library Service Workflow

Advantages of Yeast Surface Display Library Service

-Technical experts direct service, to meet different testing needs

-Highly accurate analytical instruments, experienced operators and strict quality control ensure accurate and reliable results.

High testing efficiency and real-time monitoring of the testing process

-Higher sensitivity and lower sample consumption.

-Shorter cycle time, saving customers' waiting time.

A true one-stop service: from library construction to positive clone screening, which improves efficiency and avoids errors, thus providing our customers with objective results.

Recommend Services

Enzyme Yeast Demonstration Service: KMD Bioscience specializes in providing enzyme yeast demonstration service, offering solid technical support in the research areas of enzyme activity identification, functional enzyme screening, efficient enzyme extraction and purification, as well as in vitro directed evolution of enzymes. Our services empower biopharmaceutical R&D projects and ensure that our customers achieve significant results in their downstream R&D efforts.

Yeast Expression Systems: KMD Bioscience provides one-stop service for yeast expression systems, using hosts such as Saccharomyces cerevisiae or Pichia pastoris, with a team of professionals optimizing the conditions to ensure efficient protein expression for a wide range of applications in protein production, drug discovery and vaccine preparation.

Yeast hybridization service: KMD Bioscience provides yeast hybridization service, using advanced SMART technology to construct libraries, combining selected yeast strains, precise reporter gene systems and efficient expression vectors to form a comprehensive and efficient research tool. This system provides powerful technical support for exploring protein interactions.

Reference

[1] WELLNER A,MCMAHON C,GILMAN MSA,CLEMENTS JR,CLARK S,NGUYEN KM,HO MH,HU VJ,SHIN JE,FELDMAN J,HAUSER BM,CARADONNA TM,WINGLER LM,SCHMIDT AG,MARKS DS,ABRAHAM J,KRUSE AC,LIU CC.Rapid generation of potent antibodies by autonomous hypermutation in yeast[J].Nature Chemical Biology,2021,17(10):1057-1064.

[2] GRAY A,BRADBURY ARM,KNAPPIK A,PLÜCKTHUN A,BORREBAECK CAK,DÜBEL S.Animal-free alternatives and the antibody iceberg[J].Nature Biotechnology,2020,38(11):1234-1239.

[3] BOWLEY DR,LABRIJN AF,ZWICK MB,BURTONDR.Antigen selection from an HIV-1 immune antibody library displayed on yeast yields many novel antibodies compared to selection from the same library displayed on phage[J].Protein Engineering,Design and Selection,2007,20(2):81-90.

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-022-8216-4980;