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| Catalog Number | KMP2245 |
|---|---|
| Product Name | Human CD73 Protein, Fc Tag |
| Product Description | The Human CD73 Protein(KMP2245) is produced in HEK293 Cells and the target gene encoding Trp27-Lys547 is expressed with a Fc tag at the C-terminus. |
| Molecular Weight | 57.79 kDa |
| Alias | 5'-Nucleotidase, NT5E |
| Species | Human |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purification | Affinity purification |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Buffer | 20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH7.5 |
| Uniprot | P21589 |
| SDS-PAGE |  |
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). |
| Background | CD73 is a glycosyl phosphatidylinositol(GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries(CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer. |
| Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Note | This product is for research use only. |
| References | 1.Biochem. J. 441:131-141 (2012) 2.ChemBioChem 13:2384-2391 (2012) 3.Structure 20:2161-2173 (2012) 4.PLoS ONE 9:E98568-E98568 (2014) 5.Purinergic Signal. 17:693-704 (2021) |
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