Service Line:+1-315-239-3085
Address:FL-4, Building A5, International Enterprise Community, Tianjin, China
Email:info@kmdbioscience.com
Catalog Number | KMP2784 |
---|---|
Product Name | Human CD73 Protein, His Tag |
Product Description | The Human CD73 Protein(KMP2784) is produced in HEK293 Cells. The target gene encoding the human NT5E (NP_002517.1) (Met1-Lys547) is expressed, fused with a polyhistidine tag at the C-terminus. The purified protein is biotinylated in vitro. |
Molecular Name | CD73 |
Alias | Human CD73 Protein, His Tag, CD73 |
Molecular Weight | 59.2 kDa |
Species | Human |
Size | 50ug, 100ug, 200ug |
Host | HEK293 Cells |
Purity | >85% as determined by SDS-PAGE |
Endotoxin | <1.0 EU/ug determined by the LAL method |
Buffer | PBS, pH7.4 |
Purification | Affinity purification |
Background | 5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium. |
Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Note | This product is for research use only. |