KMD Bioscience Co., Ltd.
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| Catalog Number | KMP2355 |
|---|---|
| Product Name | Human CD274 Protein, Flag Tag |
| Product Description | The Human CD274 Protein(KMP2355) is produced in HEK293 Cells and the target gene encoding Phe19-Thr239 is expressed with a Flag tag at the C-terminus. |
| Molecular Weight | 26.29 kDa |
| Alias | programmed cell death 1 ligand 1, CD274 |
| Species | Human |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purification | Affinity purification |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Buffer | 20mM PB, 150mM NaCl, pH7.4 |
| Uniprot | Q15116 |
| SDS-PAGE |  |
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 . Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival . The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function . The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy . |
| Background | CD274, also known as B7-H1 or programmed death ligand 1(PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1(PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers. |
| Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Note | This product is for research use only. |
| References | 1.Ann. N. Y. Acad. Sci. 1217:45-59 (2011) 2.Gene 638:20-25 (2018) |
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