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Catalog Number | KMP3251 |
---|---|
Product Name | Human ATP6V1F Protein, GST Tag |
Product Description | The Human ATP6V1F Protein(KMP3251) is produced in E. coli. The target gene encoding the human ATP6V1F (NP_004222.2) (Met1-Arg119) is expressed with the GST tag at the N-terminus. |
Molecular Name | ATP6V1F |
Alias | Human ATP6V1F Protein, GST Tag, ATP6V1F |
Molecular Weight | 40.2 kDa |
Species | Human |
Size | 50ug, 100ug, 200ug |
Host | E. coli |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | <1.0 EU/ug determined by the LAL method |
Buffer | PBS, pH7.4 |
Purification | Affinity purification |
Background | ATP6V1F encodes a component of vacuolar ATPase mediating acidification. The cDNA and the genomic sequences of ATP6V1F were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction and touchdown-polymerase chain reaction, respectively. Topology prediction showed that there is one protein kinase C phosphorylation site, two Casein kinase II phosphorylation sites, and one N-myristoylation site in the ATP6V1F protein. Up-regulated expression of mammary tumor 8 kDa protein (MAT-8), complement component C1S (C1S), ferritin heavy chain (FTH1), peptidyl-prolyl cis-trans isomerase A (PPIA), RNA-binding protein regulatory subunit DJ-1 protein (DJ-1) and vacuolar ATP synthase subunit F (ATP6V1F) was determined in prostate carcinoma and confirmed by using quantitative real-time RT-PCR analyses. |
Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Note | This product is for research use only. |