KMD Bioscience Co., Ltd.
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Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
| Catalog Number | PA177 |
|---|---|
| Product Name | Human respiratory syncytial virus Fusion glycoprotein F0, His tag |
| Product Description | A DNA sequence encoding the human respiratory syncytial virus Fusion glycoprotein F0 (Met1-Asn524) was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | RSV-F |
| Alias | F Protein, RSV; HRSVgp08 Protein, RSV |
| Size | 100ug |
| Species | RSV |
| Host | Baculovirus-Insect Cells |
| Conjugate | HIS |
| Predicted Molecular Weight | 75.98 and 61.80 KDa |
| Purity | > 95 % as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Buffer | Lyophilized from 20mM Tris, 300mM NaCl, 10% glycerol, pH7.5. |
| Purification | Affinity Purification |
| Background | Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis. |
| Storage | Store it under sterile conditions at -80℃. |
| Shipping Condition | Ice packs. |
| Note | For reasearch use only. |
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
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