Protein Nucleic Acid Aptamer Screening Service

The research team of KMD Bioscience has rich experience in antibody discovery, nucleic acid aptamer screening, affinity determination, and other fields. The nucleic acid aptamer screening service provided by KMD Bioscience can quickly and accurately screen aptamers with high specificity for customers, saving costs for their experimental projects. Customers only need to provide the relevant sequence of the target protein, and KMD Bioscience uses the SELEX screening method (SELEX screening method can identify different cells or detect molecular level differences between two types of cells) to screen for adapters targeting the target protein, thereby obtaining protein aptamer sequences (including RNA, DNA, oligonucleotide sequences) specific to the target protein. In addition, KMD Bioscience can also provide customers with one-stop services, from gene analysis synthesis, in vitro screening of aptamers, aptamer synthesis, to affinity determination, to meet the needs of different customers and provide strong support for downstream research and development work such as ligand function verification and targeted development of specific molecular drugs.

KMD Bioscience can provide selex screening services based on different types of samples for aptamer development to customers (including proteins, peptides, amino acids, small molecule substances, etc.), and KMD Bioscience's aptamer library has a capacity of 10 ^ 13-10 ^ 14, which is sufficient to screen high affinity nucleic acid aptamers for customer targets. After 6-10 rounds of pressure screening using various screening methods (including magnetic bead SELEX, cell SELEX, capture SELEX, etc.), highly specific nucleic acid aptamers can be screened. After NGS sequencing, 5-15 aptamer sequences targeting the target molecule can be obtained. Based on the mature SELEX technology platform, the selected nucleic acid aptamers can have higher binding affinity than conventional aptamers, reaching the nM-pM level, and the protein aptamer affinity for protein targets can reach the pM level. The selected adapter can be used for molecular interaction analysis of proteins, nucleic acids, peptides, nanomaterials, and other molecules.

In addition, KMD Bioscience can provide downstream services for protein nucleic acid adapter screening services (including protein nucleic acid adapter synthesis, affinity determination, etc.), providing strong support for customers' experiments. Scientists at KMD Bioscience are able to analyze and design reasonable solutions based on specific experimental needs of customers, provide personalized customized services, and meet the needs of every customer.

Protein Nucleic Acid Aptamer Screening Service

Single stranded nucleic acids (DNA or RNA) obtained through in vitro screening techniques can recognize corresponding target molecules. These single stranded nucleic acids are also known as nucleic acid aptamers, and their three-dimensional structure determines their high specificity. Therefore, they can specifically bind to target molecules and are widely used in drug development. Nucleic acid aptamers are highly specific in recognizing and binding to target molecules, which enables them to accurately locate targets among numerous molecules, thereby avoiding interference caused by non-specific binding. The binding ability between nucleic acid aptamers and target molecules is strong, and their affinity can even exceed that of antibodies in some cases. This high affinity makes nucleic acid aptamers highly sensitive and accurate in fields such as detection and diagnosis. And nucleic acid aptamers are easy to prepare and modify, and can be synthesized quickly and in large quantities in the laboratory without the need for complex biological preparation processes. Nucleic acid aptamers are easy to chemically modify, such as adding fluorescent labels, radioactive isotopes, etc., to enhance their stability, specificity, or endow them with new functions. This flexibility enables nucleic acid aptamers to adapt to different application scenarios and needs. Nucleic acid aptamers have good chemical stability and can maintain their structure and function unaffected under various environmental conditions. Compared with protein molecules such as antibodies, nucleic acid aptamers have higher thermal stability and can maintain their activity at higher temperatures, thereby expanding their application range. As non protein molecules, nucleic acid aptamers have low immunogenicity and are less likely to cause immune reactions in the body. This characteristic makes nucleic acid aptamers safer and more tolerable for in vivo applications. The molecular weight of nucleic acid aptamers is relatively small, which makes them easier to penetrate cell and tissue barriers and quickly reach the site of action. Due to its small molecular weight and structural flexibility, nucleic acid aptamers can bind more effectively to target molecules, improving their efficiency and effectiveness in in vivo applications. Nucleic acid aptamers can specifically bind to target molecules for early diagnosis and screening of diseases. As a targeting molecule, nucleic acid aptamers can accurately deliver drugs to the lesion site, improve therapeutic efficacy, and reduce side effects. Biosensors combined with nucleic acid aptamers can detect biomarkers and environmental pollutants with high sensitivity.

SELEX technology provides an efficient and specific method for screening nucleic acid aptamers, promoting their application and development in various fields. KMD Bioscience utilizes SELEX technology to screen nucleic acid aptamers with high affinity for specific and target substances from a random single stranded nucleic acid sequence aptamer library. Customers only need to provide the sequence number of the target protein or protein sample (protein sample must not contain large tags, buffer must not contain free amino groups, PBS buffer must not contain Tris, Protein purity>95%, Protein 0.5-1mg, concentration 1mg/ml), KMD Bioscience will perform SDS-PAGE detection on the target protein. After creating a protein aptamer library and screening the target protein, the screened adapter will be dissociated and subjected to repeated elution screening. PCR and NGS sequencing will be performed to obtain the adapter sequence specific to the target protein. The specific process is shown in Figure 1.

Figure 1: Service Process for Nucleic Acid Aptamer Screening Based on SELEX Technology

Protein Nucleic Acid Aptamer Screening Service Workflow

Advantages of Protein Nucleic Acid Aptamer Screening Service

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