KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Protein expression in yeast is a common alternative to prokaryotic and higher eukaryotic expression. Yeast cells offer many of the advantages of producing proteins in microbes (growth speed, easy genetic manipulation, low-cost media) while providing some of the attributes of higher eukaryotic systems (post-translational modifications, reasonable spatial folding).
KMD Bioscience has a relentless pursuit of quality and we know how important it is to your research. KMD Bioscience provides comprehensive and high-quality customized recombinant protein production services with a yeast expression system. Our mature protein expression systems and advanced recombinant protein production technology support us, so we can offer industry-scale protein services to meet high-throughput and large-scale production requirements. KMD Bioscience is equipped with various fermentation tanks, bioreactors, and protein purification systems, which can provide recombinant proteins from milligrams to gram levels. In addition, we are devoted to providing one-stop services from cDNA (gene) cloning to protein purification and downstream modifications such as tag addition-removal, endotoxin testing, and removal and conjugations. We can offer flexible service solutions tailored to your needs every step of the way.
Yeast Protein Expression Service Process
Yeast Protein Expression Service Workflow
|
Steps |
Specification |
Timeline |
Deliverables |
|
Gene synthesis, Cloning, Plasmid Prep |
* Gene synthesis and Codon optimization * Subcloning into an appropriate expression vector * Plasmid Prep and DNA QC |
1 week |
* Recombinant expression plasmid |
|
Transformation of yeast expression strains |
* Transform plasmids into appropriate yeast expression strains |
2 weeks |
* Positive clone strains |
|
Screening of yeast expression strains |
* Detection of recombinant protein producation |
2 weeks |
* Positive clone strains |
|
Protein expression evaluation and optimization |
* Scale up protein expression using optimized conditions |
2 weeks |
|
|
Small-scale protein expression and purification |
* The recombinant proteins were purified by affinity chromatography, ion exchange chromatography, and gel filtration (1L yeast solution) |
1-2 weeks |
* 1~10mg recombinant protein |
|
Large-scale protein expression and purification |
* 5L yeast solution（According to client requirements） |
1-2 weeks |
* According to the client's requirements |
|
Value-added services |
* Detection of the quality of the target protein by HPLC, mass spectrometry（MS） |
1-2 weeks |
* According to the client's requirements |
Yeast Protein Expression Service Highlights
--Codon optimization: including rare codon analysis, protein hydrophilicity analysis, and prediction of transmembrane domains
--High efficiency and short cycle
--A variety of fusion tag vectors and hosts to ensure high soluble protein expression and activity
--Strict quality control
--Low endotoxin level: LAL <0.1EU/ug
--Customization: quantity, purity, tags, and endotoxin levels are customizable
--Multiple scale-up capacities, including small scale (500ml, 10L) and large scale (80L, 130L, 250L and 500L)
--One-stop services, including protein purification, protein sequencing, protein labeling and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-022-8216-4980;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Hot Line： +1-315-239-3085
Technical Support：[email protected]
