KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+86-022-82164980
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
KMD Bioscience hopes that our mammalian cell expression service can help people in the laboratory gain new insights or facilitate their work. With this in mind, KMD Bioscience has been working hard in the area of mammalian protein expression. KMD Bioscience has put a lot into R&D—investment, time, energy, and passion. In mammalian protein expression, fermentation, and purification, our scientists have much to contribute, and they will continue to contribute. Custom-tailored mammalian cell expression strategies are available. For the full-length antibody and glycoprotein biological biosimilar, the folding of expression product polypeptide chain, the disulfide bond, the presence or absence of glycosylation, and the type of glycosylation often affect the properties of the synthesis, secretion, biological activity, in vivo stability, and immunogenicity of the expressed product. The mammalian expression system is ideal for eukaryotic protein expression because it has the most complicated post-translational modifications closest to the native ones. Compared with other eukaryotic expression systems, the expression of the target gene in mammalian cells is similar to that of the native protein in the type and manner of the glycosylation and can be correctly assembled into the multi-subunit protein.
With our well-established protein expression platform and experienced researchers, KMD Bioscience can offer efficient mammalian protein expression services. We are glad to witness your research findings and advances. Our mammalian transient expression system, which mainly use CHO cells and HEK293 cells as expression hosts, can provide flexible services to meet different customer needs, including tag removal, endotoxin removal, SEC-HPLC assay, glycosylation analysis, etc. Backed by various fermentation tanks, bioreactors, and protein purification systems, KMD Bioscience can provide recombinant proteins from milligram to gram level.
Mammalian Cell Expression Service Process
Mammalian Cell Expression Service Workflow
|
Steps |
Specification |
Timeline |
Deliverables |
|
Gene cloning and vector construction |
* codon optimization & gene synthesis * sequencing |
1-2 weeks |
* Sequencing data * Experimental scheme |
|
Transient mammalian cell expression |
* Transfect cells (CHO, HEK293) with expression constructs |
1-2 weeks |
* Expression results |
|
Stable mammalian cell lines (Optional) |
* Construction and screening of CHO stable expression cell lines |
4-6 weeks |
|
|
Small-scale protein expression |
* Mammalian cell culture expansion |
1-2 weeks |
* Optimized report * Culture conditions * 1-5 mg recombinant protein |
|
Large-scale protein expression and purification |
* The recombinant proteins were purified by affinity chromatography, ion exchange chromatography and gel filtration (1L), ≥90% purity |
1-2 weeks |
* 1-5mg recombinant protein |
Mammalian Cell Expression Service Highlights
--Extensive experience in mammalian protein expression
--Codon optimization: including rare codon analysis, protein hydrophilicity analysis and prediction of transmembrane domains
--High efficiency and short cycle
--Strict quality control to ensure high-level expression of both intracellular and secreted recombinant proteins
--A variety of fusion tag vectors and hosts to ensure high soluble mammalian protein expression and activity
--High-throughput and large-scale mammalian protein expression and production
--Various scale-up capacities ranging from 10 to 500 L
--Low endotoxin level: LAL <0.1EU/ug
--One-stop services, including mammalian cell culture, mammalian protein expression, protein purification, protein sequencing, protein labeling and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-022-8216-4980;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Hot Line： +86-022-82164980
Technical Support：[email protected]
