KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+86-022-82164980
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
T cell and macrophage immortalization has always been a research hotspot. KMD Bioscience has extensive experience in immortalized cell lines. We have successfully immortalized T cells and macrophages derived from goat, mouse yak, etc. We have innovated a viral transfection technology with a high success rate. Equipped with advanced technologies and an experienced scientific team, KMD Bioscience can offer excellent solutions for T cell immortalization and macrophage immortalization, helping you obtain the desired results. This enables us to guarantee a high quality of service to deliver maximum quality and flexibility in all phases of the service lifecycle.
SV40 T antigen immortalization:
Immortalized T cells and macrophages often help study tumors. At present, the methods of immortalization of T cells and macrophages mainly include SV40 T antigen immortalization and EBV immortalization. SV40 is a monkey kidney cell virus discovered and isolated in the early 1960s, and human is its natural host. SV40 has been widely used as an experimental model for mammalian replication and gene expression studies, and it can be a "probe" for high-efficiency cell function, which is associated with cell proliferation and the emergence of a variety of transformational phenotypes with tumors.
SV40 T antigen immortalization is a common method for immortalizing T cells and macrophages. It can alter the cell life cycle using its gene expression products, resulting in the formatting of immortalized bone marrow T cell-like lines.
Fig 1. T Cell/Macrophage Immortalization
Cell transformation caused by the SV40 virus is a complex process. It is generally believed that the transforming protein encoded by the SV40 virus acts on the plasma membrane of cells to change cell growth, and the transforming protein also acts on the nucleus to regulate DNA replication and gene expression.
T Cell Immortalization
T cell immortalization refers to the process of extending the lifespan and proliferative capacity of T cells, which are a type of white blood cell involved in the immune response. Normally, T cells have a limited lifespan and undergo programmed cell death (apoptosis) after completing their immune functions. However, through various techniques, it is possible to bypass this natural limitation and establish an immortalized T-cell line that can be cultured and propagated indefinitely in the laboratory. In addition to SV40 T antigen immortalization and EBV immortalization mentioned above, there are also the following methods for T cell immortalization:
--hTERT Immortalized T Cells
The human telomerase reverse transcriptase (hTERT) gene is introduced into primary T cells, typically using viral vectors, to maintain telomere length and prevent cellular senescence. hTERT extends telomeres, counteracting the natural shortening that occurs with each cell division and allowing for indefinite proliferation.
--Spontaneous Immortalization
Primary T cells are cultured under optimized conditions that promote long-term survival and proliferation, allowing rare spontaneous events to lead to immortalization. Spontaneous immortalization can occur due to genetic mutations, epigenetic changes, or adaptation to culture conditions.
--Telomerase Immortalized Cell Lines
Telomerase overexpression is induced in primary T cells to maintain telomere length and enable indefinite proliferation. Similar to hTERT Immortalized T cells, telomerase immortalization prevents telomere shortening and cellular senescence.
Common sources of immortalized t cell lines include human T cells, mouse T cells, non-human primate T cells, and other animal species such as rat, rabbit, or pig T cells.
Macrophage immortalization:
Macrophage immortalization refers to the process of extending the lifespan and proliferative capacity of macrophages, which are a type of immune cell involved in phagocytosis, antigen presentation, and immune regulation. Immortalized macrophage cell lines provide a continuous and easily accessible source of macrophages for research purposes, allowing for the study of macrophage biology, immune responses, and disease mechanisms.
Common species sources of immortalized macrophage cell lines include human macrophages, mouse macrophages, non-human primate macrophages, and other animal species such as rat, rabbit, or pig macrophages.
T Cell Immortalization and Macrophage Immortalization Service Content:
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Customer Provides:
-- Isolated cells, at least 4 tubes, the number of cells in each tube > 106
T Cell Immortalization and Macrophage Immortalization Service Highlights:
-- Immortalized cell lines from any species or tissues with the function you need
-- Reliable lab report with timely update
-- Characterization according to your requirements
-- Fast turnaround time
-- Affordable price with the best quality
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-400-621-6806.
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Hot Line： +86-022-82164980
Technical Support：[email protected]
