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B Cell Immortalization Service

Since it was recognized that the Epstein-Barr Virus (EBV) could be used in vitro, to infect B cells and produce immortalized polyclonal B cell lines in culture, B cell immortalization has always been a research hotspot. B cells have a limited life in vitro culture and cannot proliferate indefinitely. However, the immortalization of B cells fundamentally solves the problem. B cell immortalization methods mainly include human B cell hybridoma technology, EBV transformation, lentiviral-mediated genetic modification, and in vitro induced culture of CD40L combined with multiple cytokines. Each method can be used alone or in parallel. Based on the leading-edge platform and profound knowledge in cell immortalization, the specialized and dedicated team at KMD Bioscience provides excellent solutions for B cell immortalization and helps you obtain the desired results. Our advanced technologies and highly qualified personnel guarantee that we can deliver maximum quality and flexibility in all phases of the service lifecycle.

 

EBV Infection:

 

Immortalization of B cells by EBV is an effective procedure for inducing the long-term growth of certain B cells. The process can be divided into three stages: virus preparation, preparation of the target cells to be immortalized, and EBV infection and growth of infected cells. Infection of primary B cells with EBV gives rise to growth-transformed and immortalized lymphoblastoid cell lines (LCL) in vitro. Among their many applications is the use of LCL to present antigens in a variety of immunologic assays and to generate human monoclonal antibodies.

EBV's hosts are highly targeted, which tend to infect only B cells in humans and certain primates, limiting its use in other species. KMD Bioscience has successfully immortalized B cells derived from humans, rats, mice and pigs by using this technology.

 

Fig 1. Immortalization of EBV-infected B cell

 

Applications of Immortalized B Cells:

 

The EBV-based immortalization technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As a result, EBV immortalized B cell lines are extensively used as a source of biological material for functional and molecular studies and represent a potentially limitless source of genomic DNA, expression RNA, and protein and system biology analysis. In addition, these cell lines can be used as a culture model for various studies, including functional and serological studies, molecular and genetic research, large-scale biosimilar library screening, vaccine studies, and high-throughput biological studies.

 

Service Content:

 

-- Viral transduction of primary cells

-- Immortalized cell line construction services

-- Sorting of immortalized cells by flow cytometry

-- Cell line quality control (reproduce for 12 to 15 generations)

-- Cell proliferation, frozen storage and delivery

 

Customer Provides:

 

-- Isolated PBMC cells, at least 4 tubes, the number of cells in each tube > 106

 

Service Highlights:

 

-- Immortalized cell lines from any species and any tissues with the function you need

-- Reliable lab report with timely update

-- Characterization according to your requirements

-- Fast turnaround time

-- Affordable price with the best quality

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806.