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Co-immunoprecipitation Experiment

KMD Bioscience has been committed to protein interaction research for 10 years. Protein interactions can reveal the interaction patterns between molecules, effectively reveal the transduction process of signaling pathways in vivo, and are of great significance to the regulation of cells and their signals. Our research experts have accumulated many years of experience in protein interaction research and testing, and with mature technology, we can quickly complete the experimental content, saving valuable time and money for our customers. KMD Bioscience has rich experience in recombinant protein expression. Recombinant proteins, with GST, Myc-tag, Flag-tag, HA-tag and other tags can be efficiently expressed in both prokaryotic and eukaryotic expression systems. KMD Bioscience has sophisticated testing equipment and mature technology that are from Kyoto University, Japan. The co-immunoprecipitation and Pull Down technologies of KMD Bioscience come from Kyoto University's IPS laboratory in Japan, which ensures the accuracy of the experiments. KMD Bioscience, which insists on providing customers with high quality services, is able to provide one-stop technical services from recombinant protein preparation to pull down protein interactions detection to meet the needs of different customers.

Co-immunoprecipitation (Co-IP) technique is a classical method used to study protein-peptide and protein-protein interactions based on specific immunoreactivity between antibodies and antigens, and it is an effective method for determining the physiological interactions between proteins and peptides, and proteins and proteins in intact cells. The technique utilizes specific binding between antibodies and antigens, which specifically recognizes and binds to antigens in the sample, and then agarose cross-linked protein A/G specifically binds to the antibody, resulting in precipitation of the antigen-antibody complex. After elution and separation by gel electrophoresis, the amount of specific antigen in the sample is analyzed quantitatively or qualitatively by Western Blot. Immunoprecipitation verifies the natural binding of the two proteins in the cell, which is consistent with the actual situation in the body and provides high confidence in the results.

Immunoprecipitation experiments have many critical steps, in order to achieve the ultimate goal of the experiment, the immunoprecipitation system not only need high quality antibodies,  also needs to be strict control indicators. KMD Bioscience can provide customers with high quality immunoprecipitation technology services. Researchers, in KMD Bioscience, strictly control the quality of each key step in the process to ensure that customers can get the desired results.

 

 

Figure 1 Principle of immunoprecipitation technique

 

 

Main Experimental Procedures:

 

 

Co-immunoprecipitation Experiment

Experimental principle

*The experimental principle of the technique is that when cells are lysed under nondenaturing conditions, many of the protein-protein interactions present in intact cells are preserved. When protein A is immunoprecipitated with an antibody to protein A precured on agarose beads, protein B, which binds to protein A in vivo, is also precipitated; protein B is then detected by Western blot or LC-MS/MS, which demonstrates the interactions.

Applications

*Commonly used to verify the interaction of two known proteins

*Search for the presence of unknown proteins interacting with known proteins in the cell

Advantages

*Interacting proteins are post-translationally modified and in their natural state

*Protein interactions occur in their natural state, avoiding human influence

*Interacting protein complexes can be isolated in their natural state

 

Service Advantages:

 

-- Experienced experimental operation level, scientific control group setting, using high quality reagent kits for experiments, high adsorption effect of protein A magnetic beads to ensure high success rate of experiments.

-- Use of silver staining technology, higher sensitivity, SDS-PAGE electrophoresis results are clearer, more accurate and reliable results.

-- With complete supporting testing equipment, complete mass spectrometry and chromatography system, the results can be further analyzed.

-- Experienced team of experts for technical support to solve all kinds of CO-IP technical problems.

-- Complete information analysis, provide detailed experiment reports, provide co-precipitation products, SDS-PAGE results and Western Blot results, can provide protein identification results (mass spectrometry results).

-- With mature protein platform, antibody preparation and other platforms, we can provide a real one-stop service experience, saving customers' valuable time.

 

How to Order?

 

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806