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| Catalog Number | KMP2429 |
|---|---|
| Product Name | Mouse CD40L Protein, His Tag |
| Product Description | The Mouse CD40L Protein(KMP2429) is produced in HEK293 Cells and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus. |
| Molecular Weight | 17.24 kDa |
| Alias | Tumor Necrosis Factor Ligand Superfamily Member 5, CD154 |
| Species | Mouse |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purification | Affinity purification |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Buffer | 20mM PB, 200mM NaCl, 0.1mM EDTA, pH7.0 |
| Uniprot | P27548 |
| SDS-PAGE |  |
| Function | Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165). Involved in immunoglobulin class switching (PubMed:1374165). |
| Background | CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid(aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis. |
| Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Note | This product is for research use only. |
| References | Nature 357:80-82 (1992) |
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