KMD Bioscience Co., Ltd.
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| Catalog Number | KMP2532 |
|---|---|
| Product Name | Human CD40L Protein, hFc Tag, Avi Tag |
| Product Description | The Human CD40L Protein(KMP2532) is produced in HEK293 Cells. The target gene encoding the human CD40LG (NP_000065.1) (Met113-Leu261) is expressed with a n-terminal Fc region of human IgG1 tagged Avi tag at the N-terminus. The expressed protein is biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Molecular Name | CD40L |
| Alias | Human CD40L Protein, hFc Tag, Avi Tag, CD40L |
| Molecular Weight | 44.7 kDa |
| Species | Human |
| Size | 50ug, 100ug, 200ug |
| Host | HEK293 Cells |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Buffer | 20mM Tris, 150mM NaCl, pH8.5 |
| Purification | Affinity purification |
| Background | The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behAvior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation. |
| Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Note | This product is for research use only. |
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Email：info@kmdbioscience.com
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