KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
The phage display platform provided by KMD Bioscience is an ultra-high throughput ligand screening platform. The phage display platform can construct up to 1013/ml peptides or antibody clones displayed on phage. After subsequent rounds of biopanning, KMD Bioscience can provide up to 105 validated peptide or antibody sequences, providing a solid research foundation for searches of subsequent enzyme substrate, protein ligand and high-specificity antibody. Based on the phage display platform, KMD Bioscience can build 7 peptide library, 12 peptide library and cyclic 7 peptide library, etc. for customers.
Applications of 7 Phage Display Peptide Library：
Tumor-targeting therapy can be an efficacious way to cure a malignant tumor in clinical trials. Phage display is a molecular diversity technology that allows the presentation of a large number of peptides or proteins on the surface of filamentous phage for various applications. The study reported on using phage display to generate peptide libraries that bind to colon cancer tissues. To accomplish this, they developed a screening protocol that contained 3 rounds of in vitro positive panning on colon cancer cells (SW480) and 2 rounds of subtractive screening in vitro on normal human intestinal epithelial cells with a phage display-7 peptide library. After several rounds of panning, both phage titer and recovery efficiency were significantly improved. Through a cell-based enzyme-linked immunosorbent assay, immunofluorescence, in vivo binding assay, immunocytochemical staining, and immunohistochemical staining, peptide CP15 (VHLGYAT) was demonstrated to be the most effective peptide in targeting tumor cells (SW480 and HT29 cells) and tumor tissues but not the normal human intestinal epithelial cells and control colon tissue. These studies suggest that peptide CP15 may be a promising lead candidate in the development of a useful colon tumor diagnostic and targeted medicine delivery agent.
Construction of the 7 Phage Display Peptide Library Provided:
Random oligonucleotide fragments of the 7-mer peptide were synthesized by chemical synthesis and then inserted into the gene encoding the shell protein of the phage vector and transformed into E. coli by genetic engineering recombinant technology. A random 7-mer peptide was fused to the M13 phage minor coat protein (pIII) to form a combinatorial library. The 7-mer peptide shown is expressed at the N terminus of pIII, i.e., the first amino acid of the mature protein is the first amino acid of the random 7-mer peptide, and the 7-mer peptide is followed by a short stretch of spacer polypeptide consisting of Gly-Gly-Gly-Ser followed by the wild-type pIII protein. The proliferated and generated phages carry exogenous peptides on their surface, and each phage expresses one exogenous peptide on its surface. All these phages constitute a prefabricated random 7 peptide library.
The target molecule provided by the customer is used to obtain a phage clone that can bind to the target molecule with high affinity after a screening, amplification and selection step from the whole prefabricated phage random 7 peptide library. By determining the DNA sequence of this phage, the amino acid sequence of the peptide carried by the phage can be known.
Phage Display Peptide Library Screening:
Phage display describes a selection technique in which a library of peptide or protein variants is expressed on the outside of a phage virion, while the genetic material encoding each variant resides on the inside. This creates a physical linkage between each variant protein sequence and the DNA encoding it, which allows rapid partitioning based on binding affinity to a given target molecule (antibodies, enzymes, cell-surface receptors, etc.) by an in vitro selection process called panning. In its simplest form, panning is carried out by incubating a library of phage-displayed peptides on a plate (or bead) coated with the target, washing away the unbound phage, and eluting the specifically bound phage (Fig. 1). The eluted phages are then amplified and taken through additional binding/amplification cycles to enrich the pool in favor of binding sequences. After 3-4 rounds, individual clones are characterized by DNA sequencing and binding assays.
Fig. 1 Phage display peptide library screening
7 Phage Display Peptide Library Screening Service Process:
|
Services Process |
Service Content |
Service Time |
|
Support Multiple Provision Forms |
-Purified protein -Unpurified protein (provides purification services)
-Targeted targets |
|
|
Quality Control of Antigens |
Antigen purity testing by SDS-PAGE |
1 week |
|
Peptide Library Screening |
Positive clones recognizing antigen by 3-4 rounds of screening |
2 weeks |
|
Antibody Sequencing |
Positive clone sequencing |
1 week |
|
Antibody Quality Control |
Expression of target antibody in E.coli, followed by quality control by Elisa |
1 week |
|
Delivery |
Experimental report and
>10 independent peptide clones |
Depends on express |
Advantage of Service：
-- One-Stop Solutions for your research in immunology, proteomics and medicine discovery.
-- Premade phage display peptide library constructed by KMD Bioscience for high throughput production.
-- Accurate and fast experimental process, saving time for customers.
-- Professional technical experts to control the screening process accurately.
-- Screening a large number of different clones without immunogenicity problems.
-- High specificity and high sensitivity.
-- Widely used in the development of peptide medicines and accurate localization of antigenic determinants.
-- Rapid production capability of soluble antigen: combined with our existing recombinant protein expression systems, we can produce high purity and high biological activity soluble antigens to support immunization or screening of target molecules.
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com