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Peptide Library Screening Service

KMD Bioscience is offering our expertise in the phage display antibody library construction service for customers' research and development efforts. In the study of protein interactions, there is an urgent need for high-throughput screening services to meet the needs of a large number of researchers looking for protein or enzyme ligands. 

The phage display peptide library screening platform provided by KMD Bioscience is a high-throughput peptide screening method. Through this technology platform, up to 1013/ml peptide or antibody clones that have been displayed by phage can be constructed, and the average number of independent clones can be achieved to 109-1010/ml. After multiple rounds of peptide library screening, up to 105 effective peptide or antibody sequences can be found, which can provide a solid research foundation for the subsequent search for enzyme substrates, protein ligands, and highly specific antibody research. At the same time, with the affinity screening platform of KMD Bioscience, the affinity ranking of different peptides or antibodies and target proteins can be obtained in a short cycle of time (2-3 days), and even the determination of precise affinity can be completed (affinity determination service). Based on the phage display peptide platform, KMD Bioscience can construct a heptapeptide library (7 peptide library), dodecapeptide library (12 peptide library), and cyclic heptapeptide library (cyclic 7 peptide library), etc.

Peptide library construction by phage display technology has become a major tool of medicine discovery to identify de novo peptide ligands and explore sequence space in proteins. Based on the phage display technology, KMD Bioscience can provide customers with high throughput screening services for a wide range of customized random peptide libraries and premade peptide libraries. (Phage display peptide library screening service).

Peptide library construction and screening based on phage display technology have several obvious advantages over compound library peptide library screening. To read more, please click "Similarities and Differences between Phage Display Peptide Library Screening Service and Compound Library Screening Service".

KMD Bioscience is committed to providing high throughput screening services in antibody discovery and can customize peptide libraries of various types and applications according to customers' needs. KMD Bioscience's premade peptide libraries and customized peptide library screening platform provide beneficial tools for scientists. The application areas include peptide library screening for drug discovery, peptide screening for biomarker discovery, and peptide library screening for vaccine development. Cell receptor targeting peptide screening, peptide drug development customized services, cancer treatment peptide screening, protein interaction peptide screening, cell signaling pathway research peptide screening, functional peptide development services, bioinformatics-assisted peptide library design, specific peptide formulation screening and custom production of synthetic peptide libraries.


Phage Display Peptide Library Construction Service


Phage display peptide library construction involves creating a diverse collection of peptides displayed on the surface of bacteriophages, typically filamentous phages like M13. These libraries are widely used in various applications, including identifying peptide ligands for specific targets, epitope mapping, and drug discovery.




Fig. 1 Principle and procedure of peptide library construction


1. Design of the Peptide Library: Customers need to determine the characteristics required for the peptide library, such as peptide length and diversity. We suggest that the length of peptides is usually around 5-15 amino acids, and the unique sequences of libraries can range from thousands to billions.

2. Synthesis of Oligonucleotide Primers: Design and synthesize a set of oligonucleotide primers encoding the peptide sequence to be displayed, which must contain regions complementary to the end of the phage shell protein gene (such as pIII or pVIII) and the desired peptide sequence.

3. Insertion of Peptide-Encoding Sequences into Phage Genome: Oligonucleotide primers can be introduced into bacteriophage vectors containing genes encoding bacteriophage shell proteins through PCR amplification, ligation, or recombination methods, resulting in a library of bacteriophage vectors, each carrying a unique peptide coding sequence.

4. Transformation of Host Cells: Transform a suitable host bacterial strain (e.g., E. coli) with the phagemid library. The host cells will then produce the phage particles displaying the peptide sequences encoded by the phagemid vectors.

5. Packaging of Phage Library: Inducing host cells to produce phage particles displaying peptide libraries on their surface through temperature changes or chemical induction.

6. Collection of Phage Library: Harvest the phage particles from the culture supernatant by centrifugation and other methods.

7. Panning and Selection: Expose the phage library to the target, wash off unbound phages, and then elute and amplify the bound phage particles, enriching specific adhesives through multiple rounds of repetition. Through multiple rounds of biological screening, select phage particles that display peptides that bind to the target.

8. Characterization of Selected Phage Clones: Analyze the selected phage clones to identify the peptide sequence displayed on their surface by sequencing DNA involving phage segments or peptide sequencing of displayed peptides.


Phage Display Peptide Library Screening Service


Fig. 2 Phage display peptide library screening process


With rich experiences and well established phage display technology platform, KMD Bioscience can provide a wide range of customized peptide libraries with different lengths and characteristics to meet customers's needs. KMD Bioscience uses T7 and M13 phage systems as our featured service.




Nucleotide Sequence Synthesis

Random synthesis of nucleotide sequences of customer-specified length

Polymerization and Digestion

Polymerization and digestion of the synthesized DNA sequence

Electroconversion Libraries Preparation

ER2738 host bacteria were prepared, and then E. coli was transformed with the well-constructed recombinant plasmid

Peptide Library Size and Diversity Detection

Detection of peptide library size by colony counting and peptide library diversity by sequencing

Phage Display Peptide Library Generation

By infecting the phage packaging peptide library


Phage Display Peptide Library Screening Service Workflow



Service Content


Quality Control of Antigens

Antigen purity testing by SDS-PAGE

3 days

Peptide Library Screening

Positive clones recognizing antigen by 3-4 rounds of screening

2 weeks

Antibody Sequencing

Positive clone sequencing

1 week

Antibody Quality Control

Expression of target antibody in E.coli, followed by quality control by Elisa

1 week


Phage Display Peptide Library Screening Service Highlights


-- One-stop solutions for your research in immunology, proteomics, and medicine discovery.

-- Customized phage display peptide library constructed by KMD Bioscience for high throughput production.

-- Accurate and fast experimental process, saving time and cost.

-- Have professional technical experts control the screening process accurately.

-- Peptide screening has high specificity and high sensitivity.

-- Widely used in the development of peptide medicines and accurate localization of antigenic determinants.


How to Order?


If you have any questions regarding our services or products, please feel free to contact us by E-mail: or Tel: +86-400-621-6806;