Service Line:+1-315-239-3085
Address:FL-4, Building A5, International Enterprise Community, Tianjin, China
Email:[email protected]
For many years, KMD Bioscience has been working on recombinant protein expression and antibody development and has maintained high-quality requirements to produce clean and reproducible results for purification. Our laboratory has more than a dozen GE purification resins that enable simultaneous large-scale scanning protein purification and separation, saving you time and money.
You can customize protein purification services, including unlabeled recombinant proteins, purification, and separation of native proteins such as blood purification, enzyme purification, and peptide purification (synthetic or native purification). Our purification methods include affinity chromatography purification, HPLC purification, immunoaffinity chromatography purification, nickel column purification, and other recombinant protein or natural purification methods.
Protein Purification Process
Protein Purification Methods
Protein Purification Strategy |
Protein Purification Methods |
Protocol |
Overspeed Centrifugation |
Overspeed Centrifugation |
* The process of separating a mixture of particles of different mass or density suspended in a liquid using centrifugal force. After separating proteins/particles, the gradient is graded and collected to remove cell debris and other particles |
Chromatography |
Affinity Chromatography
|
* By utilizing specific binding interactions between molecules, specific ligands are chemically fixed or 'coupled' to solid carriers. Specific ligands attached to the chromatographic matrix will bind to the target protein, wash off non-specific binding proteins, and use a solution to wash off the target protein. |
Ion Exchange Chromatography
|
* Proteins are separated based on their charge, with anion exchange resin carrying a positive charge and cation exchange resin carrying a negative charge. Proteins are washed by gradually changing the pH or salt concentration of the buffer solution. |
|
Gel Filtration Chromatography
|
* Larger sizes will not enter the column but will pass through the channels between the beads relatively quickly, while smaller sizes will enter the column and have a longer path to separate through the column | |
High-performance Liquid Chromatography (HPLC)
|
* The chromatographic method of using high pressure to accelerate solute passing through a chromatographic column, and cleaning proteins based on hydrophobicity, achieves high-resolution and rapid separation. | |
Precipitation | Precipitation | * Ammonium sulfate precipitation is one of the most commonly used methods for protein purification and fractionation, which can separate proteins by changing their solubility at high salt concentrations. |
Electrophoresis | SDS-PAGE | * Used for separating proteins with molecular weights between 5 and 250 kDa. |
Isoelectric focusing | * Separate proteins based on differences in their isoelectric points (pI). | |
Ultrafiltration and dialysis | Ultrafiltration | * Separate the solution by passing it through a semi-permeable membrane through a concentration gradient. |
Dialysis | * The process of separating proteins from small molecules such as salts through a semi-permeable membrane |
Native Protein Purification Service
Project Name |
Protocol |
Separation and purification of native macromolecular proteins |
* Separation and purification of proteins using molecular sieves of appropriate pore size, combined with anion and cation adsorption columns and HLPC, identify and collect corresponding elution peaks. |
Separation and purification of native small molecular proteins |
* Multi-layer separation and filtration combined with molecular sieve to remove macromolecular impurities, combined with macroporous resin or reverse resin to purify small molecular proteins, such as HPLC purification. |
Separation and purification of mixed unknown protein samples |
* Separation and purification were carried out by KMD Bioscience, and identified by mass spectrometry, activity identification, etc. |
Recombinant Protein Purification Services
Project Name |
Protocol |
Deproteinization, separation, and purification of recombinant proteins |
* Commercially purified resin, such as nickel column, GST-Beads, etc., and purified with 6xHis, GST, SUMO, and MBP to obtain a fusion protein with a purity > 90%. |
Tag-free recombinant protein purification. |
* Antibody affinity purification; also can be selected similarly to the natural protein purification method, depending on the project. |
Inclusion body denaturation and renaturation purification |
* According to the different properties of the protein, urea, guanidine hydrochloride, detergent, extreme pH, etc. can be selected to ensure the monomeric state's maximum denaturation. |
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-022-8216-4980;