KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Protein isolation and purification is an essential part of proteomics research. KMD Bioscience has many years of experience in the purification and isolation of native proteins from tissue fluids, saliva, intestinal fluids, animal or human serums, and even some animal tissues, etc. Our scientists will analyze the approximate molecular weights of the natural proteins provided by our customers, buffer compositions, and background information to make specific analyses and feasible solutions.
KMD Bioscience has established a variety of protein purification systems (affinity chromatography, ion exchange, molecular sieve, hydrophobic chromatography, HPLC, nickel affinity chromatography, etc.) to choose from, providing strong support for customers' downstream experimental research. Our advanced AKTA purification system and a variety of specifications of pre-packed purification columns can be used to purify different sources of natural proteins. What’s more, more than a dozen types of GE purification matrixes have been developed to enable simultaneous large-scale protein purification, saving customers' research time and meeting customers' needs.
Native Protein Purification Service
The isolation and purification of native proteins are generally divided into three types: isolation and purification of natural large molecule proteins, isolation and purification of natural small molecule proteins, and isolation and purification of mixed unknown protein samples. Since natural proteins do not contain any affinity tags, it is difficult to meet the demand for simple one-step purification. We will mainly use ion exchange and molecular sieve as the core of the purification of natural proteins.
Fig 1. Native proteins purification
Separation and purification of natural large molecule proteins: According to the physicochemical properties of the target proteins to be purified, we will use molecular sieves with appropriate pore size for separation and purification, and then according to the pH value of the sample buffer we will use appropriate anion and cation adsorption columns to identify and collect the corresponding elution peaks.
Separation and purification of natural small molecule proteins: Firstly, remove the large molecule impurities through molecular sieve multilayer separation and filtration, then purify the small molecule compounds by using macroporous resin or inverse resin, and further identify the small molecule proteins by HPLC and MS. Due to the low content of small molecule proteins, the amount of purified proteins will be relatively small.
Separation and purification of mixed unknown protein samples: KMD Bioscience will formulate a corresponding plan for separation and purification according to the characteristics of the target proteins to be separated, and finally confirm the separated products through mass spectrometry, activity identification and other methods.
Fig 2. Experimental diagram of native proteins
Native Protein Purification Workflow
|
Process |
Description |
Timeline |
|
Pre-purification detection and purification test
|
(1) Protein detection by SDS-PAGE (2) Ion exchange chromatography (different buffer, elution conditions, salt gradients, etc.) (3) Graded precipitation of ammonium sulfate and hydrophobic chromatography (4) Molecular sieve separation and purification (5) Optimization of purification conditions (6) Identification of target bands by mass spectrometry (optional) |
2-3 weeks |
|
Protein purification and detection
|
(1) Bulk purification (2)SDS-PAGE (purity verification) (3) WB assay (depending on the project) (4)Concentration detection (UV absorption, BCA method, etc.) |
2-3 weeks |
Native Protein Sample Sources
|
Sample Sources |
Description |
Example |
|
Tissues (Animal or Plant) |
Proteins are extracted from specific organs or tissues of animals (e.g., liver, brain, heart) or plants (e.g., leaves, roots, seeds). |
Hemoglobin from red blood cells, collagen from connective tissue, chlorophyll-binding proteins from plants. |
|
Cell Cultures |
Proteins harvested from cultured cells, either primary cells (directly derived from tissues) or established cell lines (e.g., HeLa, CHO) |
Growth factors, signaling proteins, or secreted proteins |
|
Biological Fluids |
Proteins directly obtained from body fluids such as blood, urine, cerebrospinal fluid, or milk |
Serum albumin from blood plasma, immunoglobulins from serum, casein from milk |
|
Whole Organisms |
Proteins isolated from bacterial, yeast, fungal, or algae cultures in their natural forms |
Bacterial enzymes, yeast metabolic proteins |
Native Protein Purification Service Advantages
--Advanced equipment and diverse purification methods are suitable for natural proteins of different properties, such as blood purification, enzyme purification, and other natural proteins.
--Professional technical team for you to customize high-quality purification solutions, to obtain high-purity proteins that can meet the downstream experiments
--Advanced AKTA purification system and a variety of specifications of pre-packed purification columns, suitable for purifying natural proteins from different sources
--A variety of protein purification systems (affinity chromatography, ion exchange, molecular sieve, hydrophobic chromatography, HPLC, etc.) are available
--Provide comprehensive protein purification analysis reports
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-400-621-6806
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Hot Line： +1-315-239-3085
Technical Support：[email protected]
