Documents Download

Contact Us

Service Line:+1-315-239-3085

Address:FL-4, Building A5, International Enterprise Community, Tianjin, China

Email:info@kmdbioscience.com

Online Inquery

  •   
  •   
  •   
  • Refresh

Cell Proliferation or toxicity Testing Services

KMD Bioscience has been committed to cell biology technology research for many years, based on advanced technical equipment and complete platform construction, we can provide customers with cell proliferation/toxicity detection services. KMD Bioscience has experienced technicians who have been engaged in cell proliferation/toxicity testing for many years, and can analyze cell proliferation or cytotoxicity with high efficiency.

 

KMD Bioscience has established a complete cell culture platform with complete detection and analysis equipment related to animal experiments, and we can provide customers with a variety of cytology basic experiments. Relying on our own laboratory, we can undertake all kinds of cell proliferation/toxicity detection, and can provide a full set of customized technical services from cell culture, cell processing and production, image collection, data measurement and so on.

Cell proliferation is an important vital feature of an organism, and cells multiply in a dividing manner. Cell proliferation refers to the process by which cells grow and divide into two daughter cells and is an important life feature of an organism. Through division, the copied genetic material can be evenly distributed into two daughter cells, which is the basis for the growth, development, reproduction and inheritance of organisms. Single-celled organisms, which produce new individuals in the way that cells divide. Multicellular organisms, which produce new cells in the form of cell division to replenish aging and dying cells in the body; At the same time, multicellular organisms can be made from a fertilized egg, which undergoes cell division and differentiation and eventually develops into a new multicellular individual. Cell proliferation detection can indicate the proliferative activity of tumor cells, cell proliferation activity of transient/stable cell lines of target genes, and plays an important role in the screening and functional safety of small molecule compounds/peptides, the screening and functional safety of traditional Chinese medicines/intermediates and other medcine, the gene function of target gene overexpression, and the gene function of target gene RNAi interference.

Cytotoxicity is a simple cell-killing event caused by synthetic compounds, naturally occurring toxicity of cells, or immunomodulatory cells (e.g., cytotoxic T lymphocytes, natural killer cells, etc.). Unlike apoptosis and necrosis, cytotoxicity does not refer to a specific mechanism of cell death and does not depend on the mechanism of cell death of apoptosis or necrosis. Cytotoxicity analysis can be used in basic research and medcine discovery to screen toxic compound libraries. Compounds that produce cytotoxic reactions can be eliminated in subsequent screening; Alternatively, compounds targeting rapidly dividing cells can be selected as candidates for cancer therapy.

There are many commonly used cell proliferation/toxicity detection technologies, and Camed Bio uses MTT and CCK-8 to provide cell proliferation and toxicity detection services for customers. 

 

Cell Proliferation/Toxicity Testing Services — MTT Method:

 

Dimethylthiazole diphenyltetrazolium bromide salt (MTT), light yellow powder, good water solubility. It can be taken up by various living cells (except red blood cells), and reduced by succinate dehydrogenase in the mitochondria, transforming from a yellow soluble solution into insoluble blue-violet crystalline formazan particles. Under normal circumstances, dead cells do not have this function. The cells are lysed with a certain lysate and the formazan is dissolved, and the degree of cell proliferation can be inferred by measuring the absorbance value of the solution. The MTT method is simple, fast and accurate, and is a method often used in cytotoxicity tests and cell proliferation experiments.

 

Project

MTT Method Testing Services

Experimental principle

* MTT can enter the cell through the cell membrane, and the succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-violet crystalline formazan and deposit it in the cell, while dead cells have no function. Dimethyl sulfoxide can dissolve formazan in cells, and its light absorption value is determined by enzyme-linked immunodetector at a wavelength of 490nm, and the amount of MTT crystallization formed is proportional to the number of cells in a certain number range. Based on the measured absorbance value (OD value), the number of viable cells is judged

Advantage

* High sensitivity and economical price

* Easy to operate and practical     

Application

* MTT method is widely used in the activity detection of some bioactive factors, cell proliferation assay, large-scale anti-tumor drug screening, cytotoxicity test and tumor radiosensitivity measurement

 

Cell Proliferation/Toxicity Testing Services — CCK-8 Method:

 

CCK-8 (Cell Counting kit-8) reagent contains WST-8 [its chemical name: 2-(2-methoxy-4-nitrophenyl) -3-(4-nitrophenyl) -5- (2,4-disulfonic acid benzene) -2H-tetrazolium monosodium salt], which is reduced by dehydrogenase in cells to a highly water-soluble yellow formazan dye, and the number of formazan products generated is proportional to the number of viable cells. At a wavelength of 450 nm ~ 490 nm, The absorbance is determined by microplate reader, and the higher the OD value, the higher the number of viable cells, and the stronger the cell proliferation ability of the test product. Procedure of CCK-8 assay: cell seeding→ cell culture→ addition of CCK-8 reagent→ incubation → determination of absorbance. This method allows direct cell proliferation and toxicity analysis.

 

Project

CCK-8 MethodTesting Services

Experimental principle

* WST-8 is a compound similar to MTT that can be reduced by some dehydrogenases in mitochondria to produce orange-yellow color in the presence of electron-coupled reagents; The more cells proliferate, the darker the color; The greater the cytotoxicity, the lighter the color. For the same cell, the shade of color is linearly related to the number of cells. The light absorption value measured by enzyme-linked immunodetector at a wavelength of 450 nm can indirectly reflect the number of viable cells

Advantage

* Easy to use, eliminating the need to wash cells, no need for radioactive isotopes and organic solvents, and no need for reagents to dissolve

* Can be detected quickly  

* High sensitivity, even low cell densities can be measured

* Repeatability is better than MTT method, more stable

* No obvious toxicity to cells

application

CCK-8 method is widely used in the activity detection of some bioactive factors, large-scale antitumor medcine screening, cell proliferation test, cytotoxicity test and medcine susceptibility test

 

Cell Proliferation/Toxicity Detection Service Pocess:

  

 

 

Service Advantages:

 

--Advanced cell laboratory, complete cell culture platform, independent research and development of cell biology products; Short detection time, cell proliferation detection, cytotoxicity detection

--Short cell proliferation/toxicity detection time

--Perfect experimental operation level, perfect experimental conditions, strict cell experiment control system, experimenters have many years of successful experience in cell experiments, to ensure that your experiments can be well completed

--Provide detailed and complete experimental reports, including the original results of the experiment, each experimental parameter, which can be directly used for the writing of papers

--Complete instrument platform: with multi-type microscope, flow cytometer, enzyme-linked immunodetector and other important instruments, high sensitivity, wide linear range, good reproducibility

--Provide one-stop technical services from cell culture, cell staining to picture photography, data analysis and so on

 

How to Order?

 

If you are interested in our service offerings, please directly call the hotline at+86-400-621-6806 or send an email to info@kmdbioscience.com