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| Catalog Number | KMP2249 |
|---|---|
| Product Name | Human CD3E Protein, mFc Tag |
| Product Description | The Human CD3E Protein(KMP2249) is produced in HEK293 Cells and the target gene encoding Gln22-Thr48 is expressed with a mFc tag at the C-terminus. |
| Molecular Weight | 2.84 kDa |
| Alias | T-Cell Surface Glycoprotein CD3 Epsilon Chain, CD3E |
| Species | Human |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purification | Affinity purification |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Buffer | PBS, pH7.4 |
| Uniprot | P07766 |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). |
| Background | T-Cell Surface Glycoprotein CD3 ε Chain(CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like(immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex(TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs(ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
| Storage | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Note | This product is for research use only. |
| References | 1.J. Immunol. 163:25-31 (1999) 2.J. Immunol. 173:2262-2270 (2004) 3.J. Clin. Invest. 114:1512-1517 (2004) 4.Proc. Natl. Acad. Sci. U.S.A. 86:3277-3281 (1989) 5.Int. Immunol. 28:127-137 (2016) 6.Nat. Genet. 3:77-81 (1993) |
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