KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
KMD Bioscience is a professional provider of antibody development and purified antibody products and services, with years of pre-clinical research experience and production experience to provide upstream and downstream services for protein and antibody production. Whether you have a clearly defined protocol or you just have an idea for an experiment, KMD Bioscience can tailor it to your needs.
At present, there are different purification protocols for antibody purification, such as protein A/G, affinity purification, affinity ligand purification, etc. Suitable antibody protocol can be selected according to project requirements. The types of purified antibodies also include monoclonal antibody purification (mab purification), polyclonal antibody purification, and tag antibody purification such as flag tag purification and gst purification. KMD Bioscience can provide antibody purification strategies to help global scientists modify antibodies and produce highly specific and sensitive purification of antibodies.
Antibody Purification-Protein A/G Affinity Purification
Protein A is a cell wall protein isolated from S. aureus that binds to mammalian IgG via the Fc region. Protein A can be used to isolate and purify IgG and its subclasses and fragments. The Protein A agarose column is suitable for immunoprecipitation of murine IgG2a, IgG2b, IgA, human IgG1, IgG2, and IgG4.
Protein G is a cell wall protein isolated from G-type Streptococcus that binds to mammalian IgG via the Fc region. Protein G Agarose is more suitable for immunoprecipitation of murine IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit and sheep polyclonal antibodies, and human IgG1 , IgG2, IgG3 and IgG4.
Antibody Purification Main Process
Fig1 Protein A / G affinity Purification
Protein A/G Affinity Antibody Purification Packages
--The customer provides antiserum, and we will purify antibodies (IgG, IgM, IgD, IgA, and IgE) according to the customer's specific requirements
--Purification scale: μg ~g
--Quality Report: antibody concentration, SDS-PAGE analysis results
Antibody Purification - Affinity Ligand Purification
KMD Bioscience can provide a ligand chromatography column according to the antibody or antigen supplied by the customer; as shown in Figure 2, beads are agarose gel columns or magnetic beads (customized or recommended by us); linker can be added to reduce the steric effect between biomacromolecules.
Fig 2 Ligand chromatography column
Applications of Affinity Chromatography
--Purified biomacromolecules
--Concentration of dilute solution
--Storage of unstable proteins
--Remove residual contaminants from purified molecules
--Adsorption and purification of biomacromolecules without complementary ligands by immunosorbent
--Isolation of nucleic acids is an essential aspect of affinity chromatography applications
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-400-621-6806
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Hot Line： +1-315-239-3085
Technical Support：[email protected]
